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19.12.2024

A Spatiotemporal Atlas of Mouse Gastrulation and Early Organogenesis to Explore Axial Patterning and Project In Vitro Models onto In Vivo Space

Authors Harland LTG, Lohoff T, Koulena N, Pierson N, Pape C, Ameen F, Griffiths J, Theeuwes B, Wilson NK, Kreshuk A, Reik W, Nichols J, Cai L, Marioni JC, Gottgens B, Ghazanfar S Journal BioRxiv Citation bioRxiv 2024.12.12.628063. Abstract At the onset of murine gastrulation, pluripotent epiblast cells migrate through
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01.10.2024

Super-Resolution Axial Imaging for Quantifying Piconewton Traction Forces in Live-cells

Authors Wang DX, Kong DM, Enderlein J, Chen T Journal BioRxiv Citation bioRxiv 2024.10.01.615997. Abstract Cell mechanics play a pivotal role in regulating numerous biological processes. While super-resolution microscopy enables the imaging of cellular forces in the lateral dimension with sub-10-nanometer resolution, achieving comparable resolution along the axial dimension remains
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27.09.2024

Measuring 3-dimensional dynamics of membrane in living cell with metal-induced energy transfer imaging/spectroscopy

Authors Chen T, Gallea JI, Karedla N, Wang D, Enderlein J Journal BioRxiv Citation bioRxiv 2024.09.27.614973. Abstract The dynamics of cellular membranes are crucial for many essential cellular processes. Despite various methods available to study membrane dynamics, simultaneously measuring membrane diffusion and fluctuations within living cell membranes remains challenging. In
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12.09.2024

The epitranscriptomic m6A RNA modification modulates synaptic function in ageing and in a mouse model of synucleinopathy

Authors Chopra A, Xylaki M, Yin F, Castro-Hernández R, Merghani M, Grande V, Mollenhauer B, Fischer A, Outeiro TF Journal BioRxiv Citation bioRxiv 2024.09.12.612649. Abstract N6-methyladenosine (m6A) is the most abundant and conserved transcriptional modification in eukaryotic RNA, regulating RNA fate. While the functions of m6A in the development of
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03.09.2024

Chemical crosslinking extends and complements UV crosslinking in analysis of RNA/DNA nucleic acid–protein interaction sites by mass spectrometry

Authors Welp LM, Wulf A, Chernev A, Horokhovskyi Y, Moshkovskii S, Dybkov O, Neumann P, Pašen M, Siraj A, Raabe M, Göthert H, Walshe JL, Infante DA, Schwarzer ACdAP,Dickmanns A, Johannsson S, Schmitzova J, Wohlgemuth I, Netz E, He Y, Fritzemeier K, Delanghe B, Viner R, Vos SM, Oberbeckmann E,
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02.09.2024

Scattering-Based Super-Resolution Optical Fluctuation Imaging

Authors Yudovich S, Posnjak G, Shani L, Teblum E, Liedl T, Enderlein J, Weiss S Journal BioRxiv Citation bioRxiv 2024.09.02.610873. Abstract Super-resolution optical imaging has become a prominent tool in life and material sciences, allowing one to decipher structures at increasingly greater spatial detail. Among the utilized techniques in this
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27.08.2024

The amyloid oligomer modulator anle138b has disease modifying effects in a human IAPP transgenic mouse model of type 2 diabetes mellitus (hIAPP Ob/Ob mice)

Authors Albariqi MMA, Baauw SMG, Fens SPJP, Versteeg S, Ryazanov S, Leonov A, Willemen HLDM, Stathonikos N, Seychell RM, El Saghir A, Gerritsen B, Khemtemourian L, Vassallo N, Giese A, Eijkelkamp N, Griesinger C, Höppener JWM Journal BioRxiv Citation bioRxiv 2024.08.27.609850. Abstract Cytotoxic aggregates of human islet amyloid polypeptide (hIAPP)
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11.08.2024

Mechanism of Co-Transcriptional Cap-Snatching by Influenza Polymerase

Authors Rotsch AH, Li D, Dupont M, Krischuns T, Oberthuer C, Stelfox A, Lukarska M, Fianu I, Lidschreiber M, Naffakh N, Dienemann C, Cusack S, Cramer P Journal BioRxiv Citation bioRxiv 2024.08.11.607481. Abstract Influenza virus mRNA is stable and competent for nuclear export and translation because it receives a 5′
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22.07.2024

Transient Non-local Interactions Dominate the Dynamics of Measles Virus NTAIL

Authors Otteson L, Nagy G, Kunkel J, Kodis G, Zheng W, Bignon C, Longhi S, Grubmüller H, Vaiana AC, Vaiana SM Journal BioRxiv Citation bioRxiv 2024.07.22.604679. Abstract The RNA genome of measles virus is encapsidated by the nucleoprotein within a helical nucleocapsid that serves as template for both transcription and
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08.07.2024

Polycystin-1 loss of function increases susceptibility to atrial fibrillation through impaired DNA damage response

Authors Hendrickson T, Giese A, Fiedler M, Perez W, Reyes-Sanchez E, Reyes-Lozano M, Wang S, Venegas-Zamora L, Provasek V, El-Essawi A, Breitenbach I, Fakuade F, Kutschka I, Schiattarella GG, Voigt N, Valderrábano M, Altamirano F Journal BioRxiv Citation bioRxiv 2024.07.08.602618. Abstract Background: The increasing prevalence of atrial fibrillation (AF) and
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