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May 2021
Movement Disorders
Koss DJ, Campesan S, Giorgini F, Outeiro TF
May 2021
Biomedical Optics Express
Mojiri S, Isbaner S, Mühle S, Jang H, Bae AJ, Gregor I, Golami A, Enderlein J
April 2021
Molecular Therapy
Albert K, Raymundo DP, Panhelainen A, Eesmaa A, Shvachiy L, Araújo GR, Chmielarz P, Yan X, Singh A, Cordeiro Y, Palhano FL, Foguel D, Luk KC, Domanskyi A, Voutilainen MH, Huttunen HJ, Outeiro TF, Saarma M, Almeida MS, Airavaara M
April 2021
Journal of Biological Chemistry
Osman S, Mohammad E, Lidschreiber M, Stuetzer A, Bazsó FL, Maier KC, Urlaub H, Cramer P
April 2021
bioRxiv
Weish P, Lázaro DF, Palmares L, Santos PI, Stadelmann C, Höglinger GU, Rizzoli SO, Outeiro TF
April 2021
Structure
Huang B, Guo Q, Niedermeier ML, Cheng J, Engler T, Maurer M, Pautsch A, Baumeister W, Stengel F, Kochanek S, Fernández-Busnadiego R
April 2021
bioRxiv
Kolar MH, Nagy G, Kunkel J, Vaiana SM, Bock LV, Grubmüller H
April 2021
bioRxiv
Baeuerlein FJB, Pastor-Pareja JC, Fernandez-Busnadiego R
April 2021
Nature Communications
Trinkaus VA, Riera-Tur I, Martínez-Sánchez A, Bäuerlein FJB, Guo Q, Arzberger T, Baumeister W, Dudanova I, Hipp MS, Hartl FU, Fernández-Busnadiego R
April 2021
Nature Structural & Molecular Biology
Farnung L, Ochmann M, Engeholm M, Cramer P

Authors

Farnung L, Ochmann M, Engeholm M, Cramer P

Journal

Nature Structural & Molecular Biology

Citation

Nat Struct Mol Biol (2021).

Abstract

Efficient transcription of RNA polymerase II (Pol II) through nucleosomes requires the help of various factors. Here we show biochemically that Pol II transcription through a nucleosome is facilitated by the chromatin remodeler Chd1 and the histone chaperone FACT when the elongation factors Spt4/5 and TFIIS are present. We report cryo-EM structures of transcribing Saccharomyces cerevisiae Pol II−Spt4/5−nucleosome complexes with bound Chd1 or FACT. In the first structure, Pol II transcription exposes the proximal histone H2A−H2B dimer that is bound by Spt5. Pol II has also released the inhibitory DNA-binding region of Chd1 that is poised to pump DNA toward Pol II. In the second structure, Pol II has generated a partially unraveled nucleosome that binds FACT, which excludes Chd1 and Spt5. These results suggest that Pol II progression through a nucleosome activates Chd1, enables FACT binding and eventually triggers transfer of FACT together with histones to upstream DNA.

DOI

10.1038/s41594-021-00578-6
 
Pubmed Link

 

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