bioRxiv

Authors Gligonov I, Loetgering L, Tenopala-Carmona F, Hsieh CL, Gregor I, Enderlein J Journal BioRxiv Citation bioRxiv 2026.04.28.721333 Abstract Optical microscopy is fundamental to modern life-science research, yet interpreting its results requires precise modelling of point spread functions (PSFs) within complex environments. This manuscript introduces a versatile and efficient approach

Authors Rout SR, Kulke M, Droemer M, Wendel M, Cheng TC, Mauck TA, Asgari S, Nemec AA, Shein M, Sato Y, Fukai S, Witte G, Tomko Jr. RJ, Stengel F, Zacharias M, Schuetz AK, Sakata E Journal BioRxiv Citation bioRxiv 2026.04.16.718620 Abstract The multifunctional AAA+ ATPase p97/VCP is a pivotal

Authors Kenet S, Herwerth M, Askari S, Eichenseer K, Dornmair K, Qin X, Stadelmann C, Bennett JL, Hemmer B, Misgeld T Journal BioRxiv Citation bioRxiv 2026.03.13.711636. Abstract Neuromyelitis optica spectrum disorder (NMOSD) is an autoimmune CNS disease characterized by serum antibodies targeting astrocytes for complement-mediated lysis. NMOSD lesions show not

Authors Martinez de Paz JM, Mayer JL, Wanken P, Rodrigues Apgaua B, Ablitip A, Behera L, Macé E Journal BioRxiv Citation bioRxiv 2026.03.06.710089. Abstract Arousal fluctuations during wakefulness have a major impact on physiology and behavior, including perception and task performance. Arousal is also known to be a strong modulator

Authors Ramakrishna V, Gollisch T Journal BioRxiv Citation bioRxiv 2026.03.06.710066. Abstract Photoreceptor degeneration is among the leading causes of blindness and optogenetics as a potential therapeutic measure has garnered much attention over recent years. In this approach, light-sensitive molecules like Channelrhodopsin-2 (ChR2) are inserted into neurons in the retina to

Authors Wanken P, Edelman BJ, Behera L, Martinez de Paz JM, McCarthy PT, Macé E Journal BioRxiv Citation bioRxiv 2026.03.06.709845. Abstract Animals exhibit behavior in the absence of external stimuli or explicit tasks. Is the initiation of such spontaneous behavior shaped by internal brain states in a predictable manner? If

Authors Kuhle B, Krebs L, Bhatta A, Dennerlein S, Rehling P, Hillen H Journal BioRxiv Citation bioRxiv 2026.03.02.709036. Abstract The non-templated addition of the 3′CCA end is the final universal step of tRNA maturation. In humans, 3′CCA addition on nuclear- (nu-tRNA) and mitochondria-encoded tRNAs (mt-tRNA) is catalyzed by a single

Authors Albert L, Basak S, Koerner H, Oleksiievets N, Mougios N, Cotroneo ER, Frei MS, Enderlein J, Broichhagen J, Simeth NA, Tsukanov R, Opazo F Journal BioRxiv Citation bioRxiv 2026.03.02.709025. Abstract Multiplexed fluorescence imaging is limited by spectral overlap, whereas fluorescence lifetime provides an orthogonal encoding dimension. We replace genetically

Authors Cortes Silva JA, Gertig M, Centeno TP, Burkhardt S, Schutz AL, Sananbenesi F, Fischer A Journal BioRxiv Citation bioRxiv 2026.02.08.704661. Abstract Alzheimers disease (AD) displays pronounced regional heterogeneity, yet how transcriptional changes across brain regions converge into coordinated cellular and molecular programs remains unclear. Here, we integrated bulk and

Authors Gallea JI, Karedla N, Wang D, Zhao B, Chen L, Enderlein J, Chen T Journal BioRxiv Citation bioRxiv 2024.09.27.614973. Abstract The dynamic behavior of cellular membranes underpins essential biological processes, including signal transduction, intracellular trafficking, and mechanotransduction. However, simultaneously quantifying lateral molecular diffusion and vertical membrane fluctuations in live