Optogenetics and electrophysiology platform
Aims
The optogenetics and electrophysiology platform has been established at the MBExC with the aim of advancing optogenetic tool development and high-throughput recordings of cellular electrophysiology.
Technology
The heart of the platform is the patch-clamp robot SyncroPatch 384 from Nanion Technologies. Importantly, the combination with a modular optogenetic stimulation tool that can guide separate LED sources directly onto each cell as it is being patched allows for optogenetic stimulation in synchrony with high-throughput recordings. We expect these screenings not only to accelerate basic science research, but also to hold strong implications for the development of optogenetic therapies, like the future optical cochlear implant. Moreover, the high-throughput recordings of cellular electrophysiology considerably advance basic research in cardiology and beyond.
SyncroPatch 384 is an automated patch clamp system consisting of a patch clamp module integrated into the state-of-the-art pipetting robot. With 384 amplifier channels and a 384-pipetting head, all 384 cells are recorded in parallel, resulting in a throughput of up to 20,000 data points per day.
Contact
Prof. Dr. Niels Voigt
Molecular Pharmacology
University Medical Center Göttingen
niels.voigt[at]med.uni-goettingen.de
Dr. Thomas Mager
Institute for Auditory Neuroscience
University Medical Center Göttingen
thomas.mager[at]med.uni-goettingen.de
