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Latest Publications


May 2022
Journal of the American Chemical Society
Garrido-Charles A, Huet AT, Matera C, Thirumalai A, Hernando J, Llebaria A, Moser T, Gorostiza P
May 2022
Life Science Alliance
Bali B, Gruber-Dujardin E, Kusch K, Rankovic V, Moser T
May 2022
Basic Research in Cardiology
Jung P, Seibertz F, Fakuade FE, Ignatyeva N, Sampathkumar S, Ritter M, Li H, Mason FE, Ebert A, Voigt N.
April 2022
Journal of Physiology
Fakuade FE, Fauconnier J, Voigt N.
April 2022
EMBO Rep
Riemenschneider H, Guo Q, Bader J, Frottin F, Farny D, Kleinberger G, Haass C, Mann M, Hartl FU, Baumeister W, Hipp MS, Meissner F, Fernández-Busnadiego R, Edbauer D
April 2022
Cardiovascular Research
Liutkute, A, Brundel BJ, Voigt, N
April 2022
Trends in Neurosciences
Zapp SJ, Nitsche S, Gollisch T
April 2022
Chemistry Methods
Ding Y, Korchak S, Mamone S, Jagtap AP, Stevanato G, Sternkopf S, Moll D, Schroeder H, Becker S, Fischer A, Gerhardt E, Outeiro TF, Opazo F, Griesinger C, Glöggler S
April 2022
Journal of Molecular and Cellular Cardiology
Jebran AF, Tiburcy M, Biermann D, Balfanz P, Didié M, Karikkineth BC, Schöndube F, Kutschka I, Zimmermann WH
April 2022
Nature Communications
Wagdi A, Malan D, Sathyanarayanan U, Beauchamp JS, Vogt M, Zipf D, Beiert T, Mansuroglu B, Dusend V, Meininghaus M, Schneider L, Kalthof B, Wiegert JS, König GM, Kostenis E, Patejdl R, Sasse P, Bruegmann T

Authors

Wagdi A, Malan D, Sathyanarayanan U, Beauchamp JS, Vogt M, Zipf D, Beiert T, Mansuroglu B, Dusend V, Meininghaus M, Schneider L, Kalthof B, Wiegert JS, König GM, Kostenis E, Patejdl R, Sasse P, Bruegmann T

Journal

Nature Communications

Citation

Nat Commun. 2022 Apr 1;13(1):1765.

Abstract

Gq proteins are universally important for signal transduction in mammalian cells. The underlying kinetics and transformation from extracellular stimuli into intracellular signaling, however could not be investigated in detail so far. Here we present the human Neuropsin (hOPN5) for specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution. Properties and G protein specificity of hOPN5 are characterized by UV light induced IP3 generation, Ca2+ transients and inhibition of GIRK channel activity in HEK cells. In adult hearts from a transgenic animal model, light increases the spontaneous beating rate. In addition, we demonstrate light induced contractions in the small intestine, which are not detectable after pharmacological Gq protein block. All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening. Thus, we demonstrate specific Gq signaling of hOPN5 and unveil its potential for optogenetic applications.

DOI

10.1038/s41467-022-29265-w
 
Pubmed Link

 

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