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Januar 2025
Proceedings of the National Academy of Sciences of the United States of America
Chakraborty S, Martinez-Sanchez A, Beck F, Toro-Nahuelpan M, Hwang IY, Noh KM, Baumeister W, Mahamid J
Januar 2025
Nature Cell Biology
Oborská-Oplová M, Geiger AG, Michel E, Klingauf-Nerurkar P, Dennerlein S, Bykov YS, Amodeo S, Schneider A, Schuldiner M, Rehling P, Panse VG
Januar 2025
Methods in Microscopy
Basak S, Tsukanov R
Januar 2025
Nature
Jebran AF, Seidler T, Tiburcy M, Daskalaki M, Kutschka I, Fujita B, Ensminger S, Bremmer F, Moussavi A, Yang H, Qin X, Mißbach S, Drummer C, Baraki H, Boretius S, Hasenauer C, Nette T, Kowallick J, Ritter CO, Lotz J, Didié M, Mietsch M, Meyer T, Kensah G, Krüger D, Sakib MS, Kaurani L, Fischer A, Dressel R, Rodriguez-Polo I, Stauske M, Diecke S, Maetz-Rensing K, Gruber-Dujardin E, Bleyer M, Petersen B, Roos C, Zhang L, Walter L, Kaulfuß S, Yigit G, Wollnik B, Levent E, Roshani B, Stahl-Henning C, Ströbel P, Legler T, Riggert J, Hellenkamp K, Voigt JU, Hasenfuß G, Hinkel R, Wu JC, Behr R, Zimmermann WH
Januar 2025
Biorxiv
Revah O, Wolf F, Gutnick MJ, Neef A
Januar 2025
Cell Reports
Meissner-Bernard C, Jenkins B, Rupprecht P, Bouldoires EA, Zenke F, Friedrich RW, Frank T
Januar 2025
BioRxiv
Cretu C, Chernev A, Szabo CZK, Pena V, Urlaub H, Moser T, Preobraschenski J
Januar 2025
BioRxiv
Karagulyan N, Thirumalai A, Michanski S, Qi Y, Fang Q, Wang H, Ortner NJ, Striessnig J, Strenzke N, Wichmann C, Hua Y, Moser T
Januar 2025
Arxiv
Eskandari MA, Fischer J, Veyret N, Marx D, Betz T
Januar 2025
BioRxiv
Stoldt S, Maass F, Weber M, Dennerlein S, Ilgen P, Gaertner J, Canfes A, Schweighofer SV, Jans DC, Rehling P, Jakobs S

Authors

Stoldt S, Maass F, Weber M, Dennerlein S, Ilgen P, Gaertner J, Canfes A, Schweighofer SV, Jans DC, Rehling P, Jakobs S

Journal

BioRxiv

Citation

bioRxiv 2025.01.23.634455.

Abstract

Mitochondria contain their own DNA (mtDNA) and a dedicated gene expression machinery. As the mitochondrial dimensions are close to the diffraction limit of classical light microscopy, the spatial distribution of mitochondrial proteins and in particular of mitochondrial mRNAs remains underexplored. Here, we establish single-molecule fluorescence in situ hybridization (smFISH) combined with STED and MINFLUX super-resolution microscopy (nanoscopy) to visualize individual mitochondrial mRNA molecules and associated proteins. STED nanoscopy revealed the spatial relationships between distinct mRNA species and proteins such as the RNA granule marker GRSF1, demonstrating adaptive changes in mRNA distribution and quantity in challenged mammalian cells and patient-derived cell lines. Notably, STED-smFISH showed the release of mRNAs during apoptosis, while MINFLUX revealed the folding of the mRNAs into variable shapes, as well as their spatial proximity to mitochondrial ribosomes. These protocols are transferable to various cell types and open new avenues for understanding mitochondrial gene regulation in health and disease.

DOI

10.1101/2025.01.23.634455

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