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Latest Publications


Januar 2022
Nature Communications
Kleiber N, Lemus-Diaz N, Siller C, Heinrichs M, Mai MMQ, Hackert P, Richter-Dennerlein R, Höbartner C, Bohnsack KE, Bohnsack MT
Januar 2022
Communications Biology
Oleksiievets N, Sargsyan Y, Thiele JC, Mougios N, Sograte-Idrissi S, Nevskyi O, Gregor I, Opazo F, Thoms S, Enderlein J, Tsukanov R
Januar 2022
Nano Letters
Dietz J, Oelkers M, Hubrich R, Pérez-Lara A, Jahn R, Steinem C, Janshoff A
Dezember 2021
Elife
Dennerlein S, Poerschke S, Oeljeklaus S, Wang C, Richter-Dennerlein R, Sattmann J, Bauermeister D, Hanitsch E, Stoldt S, Langer T, Jakobs S, Warscheid B, Rehling P
Dezember 2021
Scientific Reports
Barbarics B, Eildermann K, Kaderali L, Cyganek L, Plessmann U, Bodemeyer J, Paul T, Ströbel P, Urlaub H, Tirilomis T, Lenz C, Bohnenberger H.
Dezember 2021
RNA Biology
Nadlera F, Lavdovskaiaa E, Richter-Dennerlein R
Dezember 2021
Frontiers in Physiology
Wegener JW, Wagdi A, Wagner E, Katschinski DM, Hasenfuss G, Bruegmann T, Lehnart SE
Dezember 2021
Nature Communications
Dankovich TM, Kaushik R, Olsthoorn LHM, Petersen GC, Giro PE, Kluever V, Agüi-Gonzalez P, Grewe K, Bao G, Beuermann S, Hadi HA, Doeren J, Klöppner S, Cooper BH, Dityatev A, Rizzoli SO
November 2021
PNAS
Eckermann M, Schmitzer B, van der Meer F, Franz J, Hansen O, Stadelmann C, Salditt T

Authors

Brüser C, Keller-Findeisen J, Jakobs S

Journal

Cell Reports

Citation

Cell Reports 37, 110000, November 23, 2021.

Abstract

In human cells, generally a single mitochondrial DNA (mtDNA) is compacted into a nucleoprotein complex denoted the nucleoid. Each cell contains hundreds of nucleoids, which tend to cluster into small groups. It is unknown whether all nucleoids are equally involved in mtDNA replication and transcription or whether distinct nucleoid subpopulations exist. Here, we use multi-color STED super-resolution microscopy to determine the activity of individual nucleoids in primary human cells. We demonstrate that only a minority of all nucleoids are active. Active nucleoids are physically larger and tend to be involved in both replication and transcription. Inactivity correlates with a high ratio of the mitochondrial transcription factor A (TFAM) to the mtDNA of the individual nucleoid, suggesting that TFAM-induced nucleoid compaction regulates nucleoid replication and transcription activity in vivo. We propose that the stable population of highly compacted inactive nucleoids represents a storage pool of mtDNAs with a lower mutational load.

DOI

10.1016/j.celrep.2021.110000
 
Pubmed Link

 

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