Communications Biology

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13.09.2024

MultiMatch: Geometry-Informed Colocalization in Multi-Color Super-Resolution Microscopy

Authors Naas J, Nies G, Li H, Stoldt S, Schmitzer B, Jakobs S, Munk A Journal Communications Biology Citation Commun Biol 7, 1139 (2024). Abstract With recent advances in multi-color super-resolution light microscopy, it is possible to simultaneously visualize multiple subunits within biological structures at nanometer resolution. To optimally evaluate
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23.05.2024

CaV1.3 channel clusters characterized by live-cell and isolated plasma membrane nanoscopy

Authors Schwenzer N, Teiwes NK, Kohl T, Pohl C, Giller MJ, Lehnart SE, Steinem C Journal Communications Biology Citation Commun Biol. 2024 May 23;7(1):620. Abstract A key player of excitable cells in the heart and brain is the L-type calcium channel CaV1.3. In the heart, it is required for voltage-dependent
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21.06.2023

Molecular and cellular evidence for the impact of a hypertrophic cardiomyopathy-associated RAF1 variant on the structure and function of contractile machinery in bioartificial cardiac tissues

Authors Nakhaei-Rad S, Haghighi F, Bazgir F, Dahlmann J, Busley AV, Buchholzer M, Kleemann K, Schänzer A, Borchardt A, Hahn A, Kötter S, Schanze D, Anand R, Funk F, Kronenbitter AV, Scheller J, Piekorz RP, Reichert AS, Volleth M, Wolf MJ, Cirstea IC, Gelb BD, Tartaglia M, Schmitt JP, Krüger
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21.01.2023

Single-cell transcriptomics reveal extracellular vesicles secretion with a cardiomyocyte proteostasis signature during pathological remodeling

Authors Schoger E, Bleckwedel F, Germena G, Rocha C, Tucholla P, Sobitov I, Möbius W, Sitte M, Lenz C, Samak M, Hinkel R, Varga ZV, Giricz Z, Salinas G, Gross JC, Zelarayán LC Journal Communications Biology Citation Commun Biol. 2023 Jan 21;6(1):79. Abstract Aberrant Wnt activation has been reported in failing
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15.09.2022

A modern automated patch-clamp approach for high throughput electrophysiology recordings in native cardiomyocytes

Authors Seibertz F, Rapedius M, Fakuade FE, Tomsits P, Liutkute A, Cyganek L, Becker N, Majumder R, Clauß S, Fertig N, Voigt N. Journal Communications Biology Citation Commun Biol 2022 Sep 15;5(1):969. Abstract Crucial conventional patch-clamp approaches to investigate cellular electrophysiology suffer from low-throughput and require considerable experimenter expertise. Automated
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11.01.2022

Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells

Authors Oleksiievets N, Sargsyan Y, Thiele JC, Mougios N, Sograte-Idrissi S, Nevskyi O, Gregor I, Opazo F, Thoms S, Enderlein J, Tsukanov R Journal Communications Biology Citation Commun Biol 5, 38 (2022). Abstract DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful super-resolution technique highly suitable for
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24.08.2021

The structure of a dimeric form of SARS-CoV-2 polymerase

Authors Jochheim FA, Tegunov D, Hillen HS, Schmitzova J, Kokic G, Dienemann C, Cramer P Journal Communications Biology Citation Commun Biol. 2021 Aug 24;4(1):999. Abstract The coronavirus SARS-CoV-2 uses an RNA-dependent RNA polymerase (RdRp) to replicate and transcribe its genome. Previous structures of the RdRp revealed a monomeric enzyme composed
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21.05.2021

Cryo-EM structure of mammalian RNA polymerase II in complex with human RPAP2

Authors Fianu I, Dienemann C, Aibara S, Schilbach S, Cramer P Journal Communications Biology Citation Commun Biol. 2021 May 21;4(1):606. Abstract Nuclear import of RNA polymerase II (Pol II) involves the conserved factor RPAP2. Here we report the cryo-electron microscopy (cryo-EM) structure of mammalian Pol II in complex with human
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30.10.2020

Absolute quantum yield measurements of fluorescent proteins using a plasmonic nanocavity.

Authors Ruhlandt D, Andresen M, Jensen N, Gregor I, Jakobs S, Enderlein J, Chizhik AI Journal Communications Biology Citation Commun Biol. 2020 Oct 30;3(1):627. Abstract One of the key photophysical properties of fluorescent proteins that is most difficult to measure is the quantum yield. It describes how efficiently a fluorophore
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