Cyganek, Lukas

Authors Knauer C, Haltern H, Schoger E, Kügler S, Roos L, Zelarayán LC, Hasenfuss G, Zimmermann WH, Wollnik B, Cyganek L Journal Molecular Therapy: Nucleic Acids Citation Mol Ther Nucleic Acids. 2024 Jan 23;35(1):102123. Abstract Gene variants in LZTR1 are implicated to cause Noonan syndrome associated with a severe and

Authors Rapöhn M, Cyganek L, Voigt N, Hasenfuß G, Lehnart SE, Wegener JW Journal American Journal of Physiology. Heart and Circulatory Physiology Citation Am J Physiol Heart Circ Physiol. 2024 Jan 5. Abstract Patient-derived induced pluripotent stem cells (iPSC) can be differentiated into atrial and ventricular cardiomyocytes to allow for

Authors Seibertz F, Rubio T, Springer R, Popp F, Ritter M, Liutkute A, Bartelt L, Stelzer L, Haghighi F, Pietras J, Windel H, Díaz I Pedrosa N, Rapedius M, Döring Y, Solano R, Hindmarsh R, Shi R, Tiburcy M, Brügmann T, Kutschka I, Streckfuss-Bömeke K, Kensah G, Cyganek L, Zimmermann

Authors Priesmeier L, Schoger E, Cyganek L, Zelarayán LC Journal Stem Cell Research Citation Stem Cell Res. 2023 Jul 5;71:103163. Abstract Towards increasing the possibility for temporal control of gene expression using CRISPR activation (a) systems, we generated homozygous human induced pluripotent stem cell (hiPSC) lines carrying a doxycycline (dox)-inducible

Authors Nakhaei-Rad S, Haghighi F, Bazgir F, Dahlmann J, Busley AV, Buchholzer M, Kleemann K, Schänzer A, Borchardt A, Hahn A, Kötter S, Schanze D, Anand R, Funk F, Kronenbitter AV, Scheller J, Piekorz RP, Reichert AS, Volleth M, Wolf MJ, Cirstea IC, Gelb BD, Tartaglia M, Schmitt JP, Krüger

Authors Busley AV, Cyganek L Journal Stem Cell Research Citation Stem Cell Res. 2023 Jun;69:103108. Abstract Patients harboring causative gene variants in RAS GTPase MRAS develop Noonan syndrome and early-onset hypertrophic cardiomyopathy. Here, we describe the generation of a human iPSC line harboring the Noonan syndrome-associated MRAS p.G23V variant by

Authors Busley AV, Kleinsorge M, Cyganek L   Journal Stem Cell Research   Citation Stem Cell Res. 2023 Apr 24;69:103105.   Abstract Activating KRAS codon 12 gene variants are known to cause severe RAS-MAPK and PI3K-AKT signaling pathway hyperactivity and are frequently involved in the development of various carcinomas. Here,

Authors Seibertz F, Sutanto H, Dülk R, Pronto JRD, Springer R, Rapedius M, Liutkute A, Ritter M, Jung P, Stelzer L, Hüsgen LM, Klopp M, Rubio T, Fakuade FE, Mason FE, Hartmann N, Pabel S, Streckfuss-Bömeke K, Cyganek L, Sossalla S, Heijman J, Voigt N Journal Basic Research in Cardiology

Authors Seibertz F, Rapedius M, Fakuade FE, Tomsits P, Liutkute A, Cyganek L, Becker N, Majumder R, Clauß S, Fertig N, Voigt N. Journal Communications Biology Citation Commun Biol 2022 Sep 15;5(1):969. Abstract Crucial conventional patch-clamp approaches to investigate cellular electrophysiology suffer from low-throughput and require considerable experimenter expertise. Automated

Authors Lyra-Leite DM, Gutiérrez-Gutiérrez Ó, Wang M, Zhou Y, Cyganek L, Burridge PW Journal Star Protocols Citation STAR Protoc. 2022 Aug 18;3(3):101560. Abstract The methods for the culture and cardiomyocyte differentiation of human embryonic stem cells, and later human induced pluripotent stem cells (hiPSC), have moved from a complex and