bioRxiv

Authors Martinez de Paz JM, Mayer JL, Wanken P, Rodrigues Apgaua B, Ablitip A, Behera L, Macé E Journal BioRxiv Citation bioRxiv 2026.03.06.710089. Abstract Arousal fluctuations during wakefulness have a major impact on physiology and behavior, including perception and task performance. Arousal is also known to be a strong modulator

Authors Ramakrishna V, Gollisch T Journal BioRxiv Citation bioRxiv 2026.03.06.710066. Abstract Photoreceptor degeneration is among the leading causes of blindness and optogenetics as a potential therapeutic measure has garnered much attention over recent years. In this approach, light-sensitive molecules like Channelrhodopsin-2 (ChR2) are inserted into neurons in the retina to

Authors Wanken P, Edelman BJ, Behera L, Martinez de Paz JM, McCarthy PT, Macé E Journal BioRxiv Citation bioRxiv 2026.03.06.709845. Abstract Animals exhibit behavior in the absence of external stimuli or explicit tasks. Is the initiation of such spontaneous behavior shaped by internal brain states in a predictable manner? If

Authors Kuhle B, Krebs L, Bhatta A, Dennerlein S, Rehling P, Hillen H Journal BioRxiv Citation bioRxiv 2026.03.02.709036. Abstract The non-templated addition of the 3′CCA end is the final universal step of tRNA maturation. In humans, 3′CCA addition on nuclear- (nu-tRNA) and mitochondria-encoded tRNAs (mt-tRNA) is catalyzed by a single

Authors Albert L, Basak S, Koerner H, Oleksiievets N, Mougios N, Cotroneo ER, Frei MS, Enderlein J, Broichhagen J, Simeth NA, Tsukanov R, Opazo F Journal BioRxiv Citation bioRxiv 2026.03.02.709025. Abstract Multiplexed fluorescence imaging is limited by spectral overlap, whereas fluorescence lifetime provides an orthogonal encoding dimension. We replace genetically

Authors Cortes Silva JA, Gertig M, Centeno TP, Burkhardt S, Schutz AL, Sananbenesi F, Fischer A Journal BioRxiv Citation bioRxiv 2026.02.08.704661. Abstract Alzheimers disease (AD) displays pronounced regional heterogeneity, yet how transcriptional changes across brain regions converge into coordinated cellular and molecular programs remains unclear. Here, we integrated bulk and

Authors Haertter D, Hauke L, Driehorst T, Nish Ki, Zimmermann W, Schmidt CF Journal BioRxiv Citation bioRxiv 2024.05.28.596183. Abstract Cardiac contraction is driven by the collective action of cardiomyocytes, that contain parallel bundles of myofibrils consisting of linear chains of sarcomeres, the basic force-generating units. The dynamics of individual sarcomeres

Authors Schuh M, Saha D, Manshaei S, Cavazza T, Holubcova S, Maierova B, Zielinska AP, Wartosch L, Blaney M, Elder K Journal BioRxiv Citation bioRxiv 2026.01.08.698387. Abstract Aneuploidy in human eggs, which rises sharply with age, is a leading cause of infertility, IVF failure, and miscarriage. This age-related aneuploidy is

Authors Basak S, Inamdar K, Pollack YG, Albert L, Jans DC, Jakobs S, Enderlein J, Tsukanov R, Opazo F Journal BioRxiv Citation bioRxiv 2025.12.17.694880 Abstract Direct STORM relies on robust fluorophore blinking; observations suggest that AlexaFluor647 underperforms when coupled to nanobodies, thereby undermining the advantages of nanobodies. We show that

Authors Roos L, Meric Diniz A, Koert E, Schilling M, Uhl M, Thirumalai A, Aakhte M, Kusch K, Huisken J, Moser T, Pape C Journal BioRxiv Citation bioRxiv 2025.11.16.688700. Abstract With the emergence of gene and optogenetic therapies targeting deafness, the comprehensive analysis of the molecular anatomy and physiology of