Enderlein, Jörg

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17.01.2024

Metal-induced energy transfer (MIET) Imaging of cell surface engineering with multivalent DNA nanobrushes

Authors Wang DX, Liu B, Han GM, Kong DM, Enderlein J, Chen T Journal ACS Nano Citation ACS Nano. 2024 Jan 17. Abstract The spacing between cells has a significant impact on cell–cell interactions, which are critical to the fate and function of both individual cells and multicellular organisms. However,
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02.01.2024

Local Water Content in Polymer Gels Measured With Super-Resolved Fluorescence Lifetime Imaging

Authors Jana S, Nevskyi O, Höche H, Trottenberg L, Siemes E, Enderlein J, Fürstenberg A, Wöll D Journal Angewandte Chemie International Edition Citation Angew Chem Int Ed Engl. 2024 Jan 2:e202318421. Abstract Water molecules play an important role in the structure, function, and dynamics of (bio-)materials. A direct access to
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16.12.2023

Observation of E-cadherin Adherens Junction Dynamics with Metal-Induced Energy Transfer Imaging and Spectroscopy

Authors Chen T, Karedla N, Enderlein J Journal BioRxiv Citation bioRxiv 2023.12.16.571977. Abstract Epithelial cadherin (E-cad) mediated cell-cell junctions play a crucial role in the establishment and maintenance of tissues and organs. In this study, we employed metal-induced energy transfer imaging and spectroscopy to investigate variations in intermembrane distance during
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24.08.2023

Doubling the resolution of single-molecule localization microscopy with image scanning microscopy

Authors Radmacher N, Nevskyi O, Gallea JI, Thiele JC, Gregor I, Rizzoli SO, Enderlein J Journal BioRxiv Citation bioRxiv 2023.08.23.554438. Abstract Single-molecule localization microscopy (SMLM) is a widely used super-resolution microscopy technique, renowned for its simplicity and impressive achievable resolution. It is typically based on a wide-field fluorescence microscope and
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18.08.2023

DNA-Based Optical Sensors for Forces in Cytoskeletal Networks

Authors Jayachandran C, Ghosh A, Prabhune M, Bath J, Turberfield AJ, Hauke L, Enderlein J, Rehfeldt F, Schmidt CF Journal ACS Applied Nano Materials Citation ACS Appl. Nano Mater. 2023. Abstract Mechanical forces are relevant for many biological processes, from wound healing and tumor formation to cell migration and differentiation.
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18.05.2023

Measuring sub-nanometer fluctuations at microsecond temporal resolution with metal-and graphene-induced energy transfer spectroscopy

Authors Chen T, Karedla N, Enderlein J Journal BioRxiv Citation bioRxiv 2023.05.16.540910. Abstract Out-of-plane fluctuations, also known as stochastic displacements, of biological membranes play a crucial role in regulating many essential life processes within cells and organelles. Despite the availability of various methods for quantifying membrane dynamics, accurately quantifying complex
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30.03.2023

Metal-Induced Energy Transfer (MIET) for Live-Cell Imaging with Fluorescent Proteins

Authors Hauke L, Isbaner S, Ghosh A, Guido I, Turco L, Chizhik AI, Gregor I, Karedla N, Rehfeldt F, Enderlein J Journal ACS Nano Citation ACS Nano. 2023 Mar 30. Abstract Metal-induced energy transfer (MIET) imaging is an easy-to-implement super-resolution modality that achieves nanometer resolution along the optical axis of
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09.03.2023

Excitation Intensity-Dependent Quantum Yield of Semiconductor Nanocrystals

Authors Ghosh S, Ross U, Chizhik AM, Kuo Y, Jeong BG, Bae WK, Park K, Li J, Oron D, Weiss S, Enderlein J, Chizhik AI Journal The Journal of Physical Chemistry Letters Citation J Phys Chem Lett. 2023 Mar 9:2702-2707. Abstract One of the key phenomena that determine the fluorescence
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27.01.2023

Cholesterol-Induced Nanoscale Variations in the Thickness of Phospholipid Membranes

Authors Chen T, Ghosh A, Enderlein J Journal Nano Letters Citation Nano Lett. 2023 Jan 27. Abstract Graphene-induced energy transfer (GIET) is a recently developed fluorescence-spectroscopic technique that achieves subnanometric optical localization of fluorophores along the optical axis of a microscope. GIET is based on the near-field energy transfer from
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06.07.2022

Single-Molecule Fluorescence Lifetime Imaging Using Wide-Field and Confocal-Laser Scanning Microscopy: A Comparative Analysis

Authors Oleksiievets N, Mathew C, Thiele JC, Gallea J, Nevskyi O, Gregor I, Weber A, Tsukanov R, Enderlein J Journal Nano Letters Citation Nano Lett. 2022. Abstract A recent addition to the toolbox of super-resolution microscopy methods is fluorescence-lifetime single-molecule localization microscopy (FL-SMLM). The synergy of SMLM and fluorescence-lifetime imaging
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