Enderlein, Jörg

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15.12.2021

Super-resolution imaging: when biophysics meets nanophotonics

Authors Koenderink AF, Tsukanov R, Enderlein J, Izeddin I, Krachmalnicoff V Journal Nanophotonics Citation Nanophotonics, vol. 11, no. 2, 2022, pp. 169-202. Abstract Probing light–matter interaction at the nanometer scale is one of the most fascinating topics of modern optics. Its importance is underlined by the large span of fields
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19.11.2021

Advanced Data Analysis for Fluorescence-Lifetime Single-Molecule Localization Microscopy

Authors Thiele JC, Nevskyi O, Helmerich DA, Sauer M, Enderlein J Journal Frontiers in Bioinformatics Citation Frontiers Bioinformatics 1, 56, (2021). Abstract Fluorescence-lifetime single molecule localization microscopy (FL-SMLM) adds the lifetime dimension to the spatial super-resolution provided by SMLM. Independent of intensity and spectrum, this lifetime information can be used,
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08.09.2021

Instant three-color multiplane fluorescence microscopy

Authors Gregor I, Butkevich E, Enderlein J, Mojiri S Journal Biophysical Reports Citation Biophysical Reports 1 (1), 8 Sep 2021, 100001. Abstract One of the most widely used microscopy techniques in biology and medicine is fluorescence microscopy, offering high specificity in labeling as well as maximal sensitivity. For live-cell imaging,
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06.08.2021

Advanced fluorescence correlation spectroscopy for studying biomolecular conformation

Authors Ghosh A, Enderlein J Journal Current Opinion in Structural Biology Citation Curr Opin Struct Biol. 2021 Aug 6;70:123-131. Abstract We present the recent developments and advances in fluorescence correlation spectroscopy (FCS) and their application to the investigation of biomolecular conformations. In particular, we present and discuss three techniques: multichannel
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24.06.2021

Photophysical properties and fluorescence lifetime imaging of exfoliated near-infrared fluorescent silicate nanosheets

Authors Selvaggio G, Weitzel M, Oleksiievets N, Oswald TA, Nißler R, Mey I, Karius V, Enderlein J, Tsukanov R, Kruss S Journal Nanoscale Advances Citation Nanoscale Adv., 2021. Abstract The layered silicates Egyptian Blue (CaCuSi4O10, EB), Han Blue (BaCuSi4O10, HB) and Han Purple (BaCuSi2O6, HP) emit as bulk materials bright
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16.06.2021

Mapping Activity-Dependent Quasi-Stationary States of Mitochondrial Membranes with Graphene-Induced Energy Transfer Imaging

Authors Raja SO, Chizhik AI, Schmidt CF, Enderlein J, Ghosh A Journal Nano Letters Citation Nano Lett. 2021, 21, 8244−8249 Abstract Graphene-induced energy transfer (GIET) was recently introduced for sub-nanometric axial localization of fluorescent molecules. GIET relies on near-field energy transfer from an optically excited fluorophore to a single sheet
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14.06.2021

Radiative Rate Modulation Reveals Near-Unity Quantum Yield of Graphene Quantum Dots

Authors Ghosh S, Oleksiievets N, Enderlein J, Chizhik AI Journal Advanced Optical Materials Citation Adv. Optical Mater. 2021, 2100314. Abstract A fundamental but difficult to assess photophysical property of quantum emitters is their fluorescence quantum yield. It describes how efficiently a fluorophore converts absorbed light into fluorescence. Conventional measurements of
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07.06.2021

Graphene- and metal-induced energy transfer for single-molecule imaging and live-cell nanoscopy with (sub)-nanometer axial resolution

Authors Ghosh A, Chizhik AI, Karedla N, Enderlein J Journal Nature Protocols Citation Nat Protoc (2021). Abstract Super-resolution fluorescence imaging that surpasses the classical optical resolution limit is widely utilized for resolving the spatial organization of biological structures at molecular length scales. In one example, single-molecule localization microscopy, the lateral
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02.06.2021

Transmembrane β-peptide helices as molecular rulers at the membrane surface

Authors Kloos M, Sharma A, Enderlein J, Diederichsen U Journal Journal of Peptide Science Citation J Pept Sci. 2021 Jun 2:e3355. Abstract β-Peptides are known to form 14-helices with high conformational rigidity, helical persistence length, and well-defined spacing and orientation regularity of amino acid side chains. Therefore, β-peptides are well
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07.05.2021

Rapid multi-plane phase-contrast microscopy reveals torsional dynamics in flagellar motion

Authors Mojiri S, Isbaner S, Mühle S, Jang H, Bae AJ, Gregor I, Golami A, Enderlein J Journal Biomedical Optics Express Citation Biomed. Opt. Express 12, 3169-3180 (2021). Abstract High speed volumetric optical microscopy is an important tool for observing rapid processes in living cells or for real-time tracking of
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