Enderlein, Jörg

Authors Albert L, Basak S, Koerner H, Oleksiievets N, Mougios N, Cotroneo ER, Frei MS, Enderlein J, Broichhagen J, Simeth NA, Tsukanov R, Opazo F Journal BioRxiv Citation bioRxiv 2026.03.02.709025. Abstract Multiplexed fluorescence imaging is limited by spectral overlap, whereas fluorescence lifetime provides an orthogonal encoding dimension. We replace genetically

Authors Gallea JI, Karedla N, Wang D, Zhao B, Chen L, Enderlein J, Chen T Journal BioRxiv Citation bioRxiv 2024.09.27.614973. Abstract The dynamic behavior of cellular membranes underpins essential biological processes, including signal transduction, intracellular trafficking, and mechanotransduction. However, simultaneously quantifying lateral molecular diffusion and vertical membrane fluctuations in live

Authors Marx D, Gligonov I, Malsbenden D, Wöll D, Nevskyi O, Enderlein J. Journal Nano Letters Citation Nano Lett. 2026 Jan 20. Abstract Single fluorescent molecules, acting as ideal point dipoles, offer a unique means to probe light-matter interactions at the nanoscale. Here, we exploit this property to map the

Authors Basak S, Vu KC, Mougios N, Oleksiievets N, Pollack YG, Brandenburg S, Opazo F, Lehnart SE, Enderlein J, Tsukanov R Journal ACS Nano Citation ACS Nano. 2026 Jan 1. Abstract DNA-based Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) is a powerful variant of single-molecule localization microscopy (SMLM) that

Authors Basak S, Inamdar K, Pollack YG, Albert L, Jans DC, Jakobs S, Enderlein J, Tsukanov R, Opazo F Journal BioRxiv Citation bioRxiv 2025.12.17.694880 Abstract Direct STORM relies on robust fluorophore blinking; observations suggest that AlexaFluor647 underperforms when coupled to nanobodies, thereby undermining the advantages of nanobodies. We show that

Authors Enderlein J Journal eLight Citation eLight 5, 34 (2025). Abstract Historically constrained by the low throughput of traditional Time-Correlated Single-Photon Counting (TCSPC), a new method utilizes an optimized, time-gated single-photon camera acquisition scheme for achieving single-molecule lifetime precision comparable to TCSPC, but across a wide field of view simultaneously.

Authors Stumpf D, Jensen N, Mittelheisser C, Keller-Findeisen J, Chizhik AI, Kamper M, Diekmann T, Habenstein F, Jansen I, Enderlein J, Sliwa M, Inamdar K, Hell SW, Jakobs S Journal Proceedings of the National Academy of Sciences of the United States of America Citation Proc Natl Acad Sci U S

Authors Gligonov I, Enderlein J Journal Biophysical Journal Citation Biophys J. 2025 Oct 21;124(20):3366-3379. Abstract Zernike polynomials are a sequence of orthogonal polynomials that play a crucial role in optics and, in particular, modeling microscopy systems. Introduced by Frits Zernike in 1934, they are particularly useful in expressing wavefront aberrations

Authors Schwenzer N, Tsukanov R, Kohl T, Basak S, Sobitov I, Seibertz F, Kapoor R, Voigt N, Enderlein J, Lehnart SE   Journal Journal of Molecular and Cellular Cardiology Plus   Citation J Mol Cell Cardiol Plus. 2025 Oct 16;14:100490.   Abstract The clustering of L-type calcium channels (LTCC) for

Authors Marx D, Gligonov I, Malsbenden D, Wöll D, Nevskyi O, Enderlein J Journal Arxiv Citation arXiv:2510.05791. Abstract Single fluorescent molecules, behaving as ideal electric dipole emitters, are powerful nanoscopic probes of complex optical fields. Here, this property is exploited to precisely map the polarization and vectorial structure of tightly