Cramer, Patrick

Home >> Publications >> Author >> Cramer, Patrick
Feature image not available
06.07.2023

ARS2 instructs early transcription termination-coupled RNA decay by recruiting ZC3H4 to nascent transcripts

Authors Rouvière JO, Salerno-Kochan A, Lykke-Andersen S, Garland W, Dou Y, Rathore O, Smidova Molska E, Wu G, Schmid M, Bugai A, Jakobsen L, Zumer K, Cramer P, Andersen JS, Conti E, Jensen TH Journal Molecular Cell Citation Mol Cell. 2023 Jul 6;83(13):2240-2257.e6. Abstract The RNA-binding ARS2 protein is centrally
Learn More
Feature image not available
26.06.2023

Structural insights into human co-transcriptional capping

Authors Garg G, Dienemann C, Farnung L, Schwarz J, Linden A, Urlaub H, Cramer P Journal Molecular Cell Citation Mol Cell 2023. in press. Abstract Co-transcriptional capping of the nascent pre-mRNA 5′ end prevents degradation of RNA polymerase (Pol) II transcripts and suppresses the innate immune response. Here, we provide
Learn More
Feature image not available
04.04.2023

Yeast PIC-Mediator structure with RNA polymerase II C-terminal domain

Authors Schilbach S, Wang H, Dienemann C, Cramer P Journal Proceedings of the National Academy of Sciences Citation Proc Natl Acad Sci U S A. 2023 Apr 11;120(15):e2220542120. Abstract For transcription initiation, RNA polymerase II (Pol II) forms a preinitiation complex (PIC) that associates with the general coactivator Mediator. Whereas
Learn More
Feature image not available
28.02.2023

In vitro reconstitution of chromatin domains

Authors Quililan K, Oberbeckmann E, Cramer P, Oudelaar AM   Journal BioRxiv   Citation bioRxiv 2023.02.27.530214.   Abstract The spatial organization of the genome modulates nuclear processes, including transcription, replication, and DNA repair1,2. Eukaryotic genomes are organized into distinct 3D chromatin domains3. However, the molecular mechanisms that drive the formation
Learn More
Feature image not available
24.11.2022

Structural basis of SNAPc-dependent snRNA transcription initiation by RNA polymerase II

Authors Rengachari S, Schilbach S, Kaliyappan T, Gouge J, Zumer K, Schwarz J, Urlaub H, Dienemann C, Vannini A, Cramer P Journal Nature Structural and Molecular Biology Citation Nat Struct Mol Biol. 2022 Nov 24. Abstract RNA polymerase II (Pol II) carries out transcription of both protein-coding and non-coding genes.
Learn More
Feature image not available
21.11.2022

Structures of transcription preinitiation complex engaged with the +1 nucleosome

Authors Wang H, Schilbach S, Ninov M, Urlaub H, Cramer P Journal Nature Structural & Molecular Biology Citation Nat Struct Mol Biol. 2022 Nov 21. Abstract The preinitiation complex (PIC) assembles on promoters of protein-coding genes to position RNA polymerase II (Pol II) for transcription initiation. Previous structural studies revealed
Learn More
Feature image not available
13.07.2022

Structure of a backtracked hexasomal intermediate of nucleosome transcription

Authors Farnung L, Ochmann M, Garg G, Vos SM, Cramer P Journal Molecular Cell Citation Mol Cell. 2022 Jul 13:S1097-2765(22)00610-4. Abstract During gene transcription, RNA polymerase II (RNA Pol II) passes nucleosomes with the help of various elongation factors. Here, we show that RNA Pol II achieves efficient nucleosome passage
Learn More
Feature image not available
27.05.2022

Oct4 differentially regulates chromatin opening and enhancer transcription in pluripotent stem cells

Authors Xiong L, Tolen EA, Choi J, Velychko S, Caizzi L, Velychko T, Adachi K, MacCarthy CM, Lidschreiber M, Cramer P, Schoeler HR Journal eLife Citation eLife 11:e71533. Abstract The transcription factor Oct4 is essential for the maintenance and induction of stem cell pluripotency, but its functional roles are not
Learn More
Feature image not available
17.05.2022

Histone H1 binding to nucleosome arrays depends on linker DNA length and trajectory

Authors Dombrowski M, Engeholm M, Dienemann C, Dodonova, S. Cramer P Journal Nature Structural & Molecular Biology Citation Nat Struct Mol Biol 29, 493–501 (2022). Abstract Throughout the genome, nucleosomes often form regular arrays that differ in nucleosome repeat length (NRL), occupancy of linker histone H1 and transcriptional activity. Here,
Learn More
Feature image not available
11.11.2021

Structural basis of Integrator-mediated transcription regulation

Authors Fianu I, Chen Y, Dienemann Ch, Dybkov O, Linden A, Urlaub H, Cramer P Journal Science Citation Science. 2021 Nov 12;374(6569):883-887 Abstract Integrator and protein phosphatase 2A (PP2A) form a complex that dephosphorylates paused RNA polymerase II (Pol II), cleaves the nascent RNA, and terminates transcription. We report the
Learn More
X
EN DE
X
X