Multi-Scale X-Ray Phase-Contrast Tomography Of Murine Heart Tissue


Reichardt M, Frohn J, Khan A, Alves F, Salditt T


Biomedical Optics Express


Biomed Opt Express. 2020 May 1; 11(5): 2633–2651.


The spatial organization of cardiac muscle tissue exhibits a complex structure on multiple length scales, from the sarcomeric unit to the whole organ. Here we demonstrate a multi-scale three-dimensional imaging (3D) approach with three levels of magnification based onsynchrotron X-ray phase contrast tomography. Whole mouse hearts are scanned in an undulatorbeam, which is first focused and then broadened by divergence. Regions-of-interest of the heartsare scanned in parallel beam as well as a biopsy by magnified cone beam geometry using a X-ray waveguide optic. Data is analyzed in terms of orientation, anisotropy and the sarcomeric periodicity via a local Fourier transformation.



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