Authors
Sakib MS, Sokpor G, Nguyen HP, Fischer A, Tuoc T
Journal
Star Protocols
Citation
STAR Protoc. 2021 Feb 2;2(1):100318.
Abstract
Cell sorting can be used to purify cell populations for cell type-specific molecular probing. Fluorescence-activated cell sorting (FACS) coupled with high-throughput sequencing affords molecular signature identification for specific cell types. FACS has many challenges that limit comprehensive cell purification from the brain, leading to incomplete molecular characterization. Here, we present the intranuclear immunostaining-based FACS protocol with several modified steps, which allows optimized nuclei/cell sorting from mouse or human embryonic cortical tissue for distinct downstream molecular investigation of basal intermediate progenitors.
DOI
10.1016/j.xpro.2021.100318
Pubmed Link
