In situ studies of membrane biology by cryo-electron tomography

Authors

Keller J, Fernández-Busnadiego R

Journal

Current Opinion in Cell Biology

Citation

Curr Opin Cell Biol. 2024 Apr 26;88:102363.

Abstract

Cryo-electron tomography (cryo-ET) allows high resolution 3D imaging of biological samples in near-native environments. Thus, cryo-ET has become the method of choice to analyze the unperturbed organization of cellular membranes. Here, we briefly discuss current cryo-ET workflows and their application to study membrane biology in situ, under basal and pathological conditions.

DOI

10.1016/j.ceb.2024.102363
 
Pubmed Link