Research Alliance

Authors Baeuerlein FJB, Pastor-Pareja JC, Fernandez-Busnadiego R Journal bioRxiv Citation bioRxiv 2021.04.14.437159. Abstract Cryo-focused ion beam (cryo-FIB) milling allows thinning vitrified cells for high resolution imaging by cryo-electron tomography (cryo-ET). However, it remains challenging to apply this workflow to tissues, as they usually require high-pressure freezing for vitrification. Here we

Authors Trinkaus VA, Riera-Tur I, Martínez-Sánchez A, Bäuerlein FJB, Guo Q, Arzberger T, Baumeister W, Dudanova I, Hipp MS, Hartl FU, Fernández-Busnadiego R Journal Nature Communications Citation Nat Commun 12, 2110 (2021). Abstract The molecular architecture of α-Synuclein (α-Syn) inclusions, pathognomonic of various neurodegenerative disorders, remains unclear. α-Syn inclusions were

Authors Farnung L, Ochmann M, Engeholm M, Cramer P Journal Nature Structural & Molecular Biology Citation Nat Struct Mol Biol (2021). Abstract Efficient transcription of RNA polymerase II (Pol II) through nucleosomes requires the help of various factors. Here we show biochemically that Pol II transcription through a nucleosome is

Authors Popova B, Wang D, Rajavel A, Dhamotharan K, Lázaro DF, Gerke J, Uhrig JF, Hoppert M, Outeiro TF, Braus GH Journal Frontiers in Molecular Neuroscience Citation Front Mol Neurosci. 2021 Apr 12;14:659926. Abstract Aggregation of α-synuclein (αSyn) into proteinaceous deposits is a pathological hallmark of a range of neurodegenerative

Authors Pein F, Eltzner B, Munk A Journal European Biophysics Journal Citation Eur Biophys J. 2021 Apr 9. Abstract Analysis of patchclamp recordings is often a challenging issue. We give practical guidance how such recordings can be analyzed using the model-free multiscale idealization methodology JSMURF, JULES, and HILDE. We provide

Authors Hillen HS Journal Current Opinion in Virology Citation Current Opinion in Virology 2021, 48:82–90. Abstract Coronaviruses use an RNA-dependent RNA polymerase (RdRp) to replicate and express their genome. The RdRp associates with additional non-structural proteins (nsps) to form a replication-transcription complex (RTC) that carries out RNA synthesis, capping and

Authors Hillen HS, Markov DA, Wojtas ID, Hofmann KB, Lidschreiber M, Cowan AT, Jones JL, Temiakov D, Cramer P, Anikin M Journal Proceedings of the National Academy of Sciences of the United States of America Citation PNAS, Apr 2021, 118 (15) e2009329118. Abstract Respiration-coupled ATP production yields most of the

Authors Hamann F, Zimmerningkat LC, Becker RA, Garbers TB, Neumann P, Hubc JS, Ficner R Journal Acta Crystallographica Section D-Structural Biology Citation Acta Cryst. (2021). D77, 496-509. Abstract Noncoding intron sequences present in precursor mRNAs need to be removed prior to translation, and they are excised via the spliceosome, a

Authors Tameling C, Stoldt S, Stephan T, Naas J, Jakobs S, Munk A Journal Nature Computational Science Citation Nat Comput Sci 1, 199–211 (2021). Abstract Super-resolution fluorescence microscopy is a widely used technique in cell biology. Stimulated emission depletion (STED) microscopy enables the recording of multiple-color images with subdiffraction resolution.

Authors Ulmke PA, Sakib MS, Ditte P, Sokpor G, Kerimoglu C, Pham L, Xie Y, Mao X, Rosenbusch J, Teichmann U, Nguyen HP, Fischer A, Eichele G, Staiger JF, Tuoc T Journal Stem Cell Reports Citation Stem Cell Reports. 2021 Mar 23:S2213-6711(21)00138-7. Abstract Intermediate progenitor cells (IPCs) are neocortical neuronal