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14.05.2020

Circumvention of common labelling artefacts using secondary nanobodies

Authors Sograte-Idrissi S, Schlichthaerle T, Duque-Afonso CJ, Alevra M, Strauss S, Moser T, Jungmann R, Rizzoli SO, Opazo F Journal Nanoscale Citation Nanoscale. 2020 May 14;12(18):10226-10239. Abstract A standard procedure to study cellular elements is via immunostaining followed by optical imaging. This methodology typically requires target-specific primary antibodies (1.Abs), which
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06.05.2020

Vesicle adhesion in the electrostatic strong- coupling regime studied by time-resolved small-angle X-ray scattering

Authors Komorowski K, Schaeper J, Sztucki M, Sharpnack L, Brehm G, Köster S, Salditt T Journal Soft Matter Citation Soft Matter. 2020 May 6;16(17):4142-4154. Abstract We have used time-resolved small-angle X-ray scattering (SAXS) to study the adhesion of lipid vesicles in the electrostatic strong-coupling regime induced by divalent ions. The
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06.05.2020

Light Microscopy of Mitochondria at the Nanoscale

Authors Jakobs S, Stephan T, Ilgen P, Brüser C Journal Annual Reviews of Biophysics Citation Annu Rev Biophys. 2020 May 6;49:289-308. Abstract Mitochondria are essential for eukaryotic life. These double-membrane organelles often form highly dynamic tubular networks interacting with many cellular structures. Their highly convoluted contiguous inner membrane compartmentalizes the
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05.05.2020

Testing for dependence on tree structures

Authors Behr M, Ansari MA, Munk A , Holmes C Journal Proceedings of the National Academy of Sciences of the United States of America Citation PNAS May 5, 2020 117 (18) 9787-9792. Abstract Tree structures, showing hierarchical relationships and the latent structures between samples, are ubiquitous in genomic and biomedical
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30.04.2020

Wide-Field Fluorescence Lifetime Imaging of Single Molecules

Authors Oleksiievets N, Thiele JC, Weber A, Gregor I, Nevskyi O, Isbaner S, Tsukanov R, Enderlein J Journal Journal of Physical Chemistry A Citation J Phys Chem A. 2020 Apr 30;124(17):3494-3500. Abstract Fluorescence lifetime imaging (FLIM) has become an important microscopy technique in bioimaging. The two most important of its applications are lifetime-multiplexing for imaging many different structures in
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24.04.2020

A cross-linking mass spectrometry approach defines protein interactions in yeast mitochondria

Authors Linden A, Deckers M, Parfentev I, Pflanz R, Homberg B, Neumann P, Ficner R, Rehling P, Urlaub H Journal Molecular & Cellular Proteomics Citation Mol Cell Proteomics. 2020 Apr 24;mcp.RA120.002028. Abstract Protein cross-linking and the analysis of cross-linked peptides by mass spectrometry is currently receiving much attention. Not only
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22.04.2020

A Transcriptome Analysis Identifies Potential Preventive and Therapeutic Approaches Towards COVID-19

Authors Islam MR, Fischer A Journal Preprints Citation Preprints 2020, 2020040399. Abstract The recent outbreak of Coronavirus Disease 2019 (COVID-19) is a major threat to human health and the global economy. In addition to the development of vaccines there is an urgent need for preventive and therapeutic strategies towards severe
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22.04.2020

Nucleosome-bound SOX2 and SOX11 structures elucidate pioneer factor function

Authors Dodonova SO, Zhu F, Dienemann C, Taipale J, Cramer P Journal Nature Citation Nature 580, 669–672 (2020). Abstract ‘Pioneer’ transcription factors are required for stem-cell pluripotency, cell differentiation and cell reprogramming1,2. Pioneer factors can bind nucleosomal DNA to enable gene expression from regions of the genome with closed chromatin.
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08.04.2020

Challenges to heart repair with pluripotent stem cell-derived cardiomyocytes

Authors Zimmermann WH Journal Trends in Cardiovascular Medicine Citation Trends Cardiovasc Med 2020 Apr 8;S1050-1738(20)30049-9 Abstract no abstract available DOI 10.1016/j.tcm.2020.03.006 Pubmed Link  
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07.04.2020

Towards the optical cochlear implant: optogenetic approaches for hearing restoration

Authors Dieter A, Keppeler D, Moser T Journal Cell Reports Citation EMBO Mol Med 2020 Apr 7;12(4):e11618. Abstract Cochlear implants (CIs) are considered the most successful neuroprosthesis as they enable speech comprehension in the majority of half a million CI users suffering from sensorineural hearing loss. By electrically stimulating the
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