Journal

Authors Aibara S, Dienemann Ch, Cramer P Journal Nucleic Acids Research Citation Nucleic Acids Research, 2021; Sep 16, gkab783 Abstract Eukaryotic gene transcription is carried out by three RNA polymerases: Pol I, Pol II and Pol III. Although it has long been known that Pol I can form homodimers, it

Authors Butola T, Alvanos T, Hintze A, Koppensteiner P, Kleindienst D, Shigemoto R, Wichmann C, Moser T Journal Journal of Neuroscience Citation J. Neurosci. 2021, 41 (37) 7742-7767 Abstract Rab-interacting molecule (RIM)-binding protein 2 (BP2) is a multidomain protein of the presynaptic active zone (AZ). By binding to RIM, bassoon

Authors Kokic G, Wagner FR, Chernev A, Urlaub H, Cramer P Journal Nature Citation Nature 2021 Abstract Transcription-coupled DNA repair removes bulky DNA lesions from the genome and protects cells against ultraviolet (UV) irradiation. Transcription-coupled DNA repair begins when RNA polymerase II (Pol II) stalls at a DNA lesion and recruits

Authors Gregor I, Butkevich E, Enderlein J, Mojiri S Journal Biophysical Reports Citation Biophysical Reports 1 (1), 8 Sep 2021, 100001. Abstract One of the most widely used microscopy techniques in biology and medicine is fluorescence microscopy, offering high specificity in labeling as well as maximal sensitivity. For live-cell imaging,

Authors Bhatta A, Dienemann C, Cramer P, Hillen HS Journal Nature Structural and Molecular Biology Citation Nat Struct Mol Biol (2021). Abstract Human mitochondrial transcripts contain messenger and ribosomal RNAs flanked by transfer RNAs (tRNAs), which are excised by mitochondrial RNase (mtRNase) P and Z to liberate all RNA species.

Authors Sadredini M, Manotheepan R, Lehnart SE, Anderson ME, Sjaastad I, Stokke MK Journal Physiological Reports Citation Physiol Rep. 2021 Sep;9(18):e15030. Abstract Catecholaminergic polymorphic ventricular tachycardia type 1 (CPVT1) is an inherited arrhythmogenic disorder caused by missense mutations in the cardiac ryanodine receptors (RyR2), that result in increased β-adrenoceptor stimulation-induced

Authors Sertel SM, Blumenstein W, Mandad S, Shomroni O, Salinas G, Rizzoli SO Journal Scientific Reports Citation Sci Rep. 2021 Aug 30;11(1):17374. Abstract A strong focus on sex-related differences has arisen recently in neurobiology, but most investigations focus on brain function in vivo, ignoring common experimental models like cultured neurons.

Authors Thom T, Schmitz M, Fischer AL, Correia A, Correia S, Llorens F, Pique AV, Möbius W, Domingues R, Zafar S, Stoops E, Silva CJ, Fischer A, Outeiro TF, Zerr I Journal Movement Disorders Citation Mov Disord. 2021 Aug 27. Abstract Background The cellular prion protein (PrPC) is a membrane-bound,

Authors Schoger E, Zimmermann WH, Cyganek L, Zelarayán LC Journal Stem Cell Research Citation Stem Cell Res. 2021 Aug 26;56:102518. Abstract CRISPR/Cas9 technology based on nuclease inactive dCas9 and fused to the heterotrimeric VPR transcriptional activator is a powerful tool to enhance endogenous transcription by targeting defined genomic loci. We

Authors Jochheim FA, Tegunov D, Hillen HS, Schmitzova J, Kokic G, Dienemann C, Cramer P Journal Communications Biology Citation Commun Biol. 2021 Aug 24;4(1):999. Abstract The coronavirus SARS-CoV-2 uses an RNA-dependent RNA polymerase (RdRp) to replicate and transcribe its genome. Previous structures of the RdRp revealed a monomeric enzyme composed