Nature Methods

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01.09.2022

DNA-PAINT MINFLUX nanoscopy

Authors Ostersehlt LM, Jans DC, Wittek A, Keller-Findeisen J, Inamdar K, Sahl SJ, Hell SW, Jakobs S Journal Nature Methods Citation Nat Methods (2022). Abstract MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations, has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on–off switching
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21.10.2021

Deep learning improves macromolecule identification in 3D cellular cryo-electron tomograms

Authors Moebel E, Martinez-Sanchez A, Lamm L, Righetto RD, Wietrzynski W, Albert S, Larivière D, Fourmentin E, Pfeffer S, Ortiz J, Baumeister W, Peng T, Engel BD, Kervrann C Journal Nature Methods Citation Nat Methods. 2021 Oct 21. Abstract Cryogenic electron tomography (cryo-ET) visualizes the 3D spatial distribution of macromolecules
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04.02.2021

Multi-particle cryo-EM refinement with M visualizes ribosome-antibiotic complex at 3.7 Å inside cells

Authors Tegunov D, Xue L, Dienemann C, Cramer P, Mahamid J Journal Nature Methods Citation Nat Methods 18, 186–193 (2021). Abstract Cryo-electron microscopy (cryo-EM) enables macromolecular structure determination in vitro and inside cells. In addition to aligning individual particles, accurate registration of sample motion and three-dimensional deformation during exposures are
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