Enderlein, Jörg

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20.08.2024

Variational calculus approach to Zernike polynomials with application to fluorescence correlation spectroscopy

Authors Gligonov I, Enderlein J Journal Biophysical Journal Citation Biophys J. 2024 Aug 19:S0006-3495(24)00557-5. Abstract Zernike polynomials are a sequence of orthogonal polynomials that play a crucial role in optics and, in particular, modeling microscopy systems. Introduced by Frits Zernike in 1934, they are particularly useful in expressing wavefront aberrations
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02.08.2024

Doubling the resolution of fluorescence-lifetime single-molecule localization microscopy with image scanning microscopy

Authors Radmacher N, Nevskyi O, Gallea JI, Thiele JC, Gregor I, Rizzoli SO, Enderlein J Journal Nature Photonics Citation Nat. Photon. (2024). Abstract In this study, we integrate a single-photon detector array into a confocal laser scanning microscope, enabling the combination of fluorescence-lifetime single-molecule localization microscopy with image scanning microscopy.
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04.04.2024

Three-dimensional multi-target super-resolution microscopy of cells using Metal-Induced Energy Transfer and DNA-PAINT

Authors Oleksiievets N, Mougios N, Jans DC, Hauke L, Thiele JC, Basak S, Jakobs S, Opazo F, Enderlein J, Tsukanov R, Sananbenesi F, Fischer A   Journal BioRxiv   Citation bioRxiv 2024.04.02.587536.   Abstract DNA-points accumulation for imaging in nanoscale topography (DNA-PAINT) is a potent variant of single-molecule localization microscopy
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27.02.2024

Measuring sub-nanometer undulations at microsecond temporal resolution with metal- and graphene-induced energy transfer spectroscopy

Authors Chen T, Karedla N, Enderlein J Journal Nature Communications Citation Nat Commun. 2024 Feb 27;15(1):1789. Abstract Out-of-plane fluctuations, also known as stochastic displacements, of biological membranes play a crucial role in regulating many essential life processes within cells and organelles. Despite the availability of various methods for quantifying membrane
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21.02.2024

Machine learning and advanced statistical analysis for Fluorescence Correlation Spectroscopy

Authors Enderlein J Journal Biophysical Journal Citation Biophys J. 2024 Feb 21:S0006-3495(24)00131-0. Abstract No abstract available. DOI 10.1016/j.bpj.2024.02.011   Pubmed Link
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17.01.2024

Metal-induced energy transfer (MIET) Imaging of cell surface engineering with multivalent DNA nanobrushes

Authors Wang DX, Liu B, Han GM, Kong DM, Enderlein J, Chen T Journal ACS Nano Citation ACS Nano. 2024 Jan 17. Abstract The spacing between cells has a significant impact on cell–cell interactions, which are critical to the fate and function of both individual cells and multicellular organisms. However,
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02.01.2024

Local Water Content in Polymer Gels Measured With Super-Resolved Fluorescence Lifetime Imaging

Authors Jana S, Nevskyi O, Höche H, Trottenberg L, Siemes E, Enderlein J, Fürstenberg A, Wöll D Journal Angewandte Chemie International Edition Citation Angew Chem Int Ed Engl. 2024 Jan 2:e202318421. Abstract Water molecules play an important role in the structure, function, and dynamics of (bio-)materials. A direct access to
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17.10.2023

Fluorophore position of headgroup-labeled Gb3 glycosphingolipids in lipid bilayers

Authors Socrier L, Sharma A, Chen T, Flato K, Kettelhoit K, Enderlein J, Werz DB, Steinem C Journal Biophysical Journal Citation Biophys J. 2023 Oct 17;122(20):4104-4112. Abstract Fluorescent lipid probes are an invaluable tool for investigating lipid membranes. In particular, localizing certain receptor lipids such as glycosphingolipids within phase-separated membranes
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18.08.2023

DNA-Based Optical Sensors for Forces in Cytoskeletal Networks

Authors Jayachandran C, Ghosh A, Prabhune M, Bath J, Turberfield AJ, Hauke L, Enderlein J, Rehfeldt F, Schmidt CF Journal ACS Applied Nano Materials Citation ACS Appl. Nano Mater. 2023. Abstract Mechanical forces are relevant for many biological processes, from wound healing and tumor formation to cell migration and differentiation.
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30.03.2023

Metal-Induced Energy Transfer (MIET) for Live-Cell Imaging with Fluorescent Proteins

Authors Hauke L, Isbaner S, Ghosh A, Guido I, Turco L, Chizhik AI, Gregor I, Karedla N, Rehfeldt F, Enderlein J Journal ACS Nano Citation ACS Nano. 2023 Mar 30. Abstract Metal-induced energy transfer (MIET) imaging is an easy-to-implement super-resolution modality that achieves nanometer resolution along the optical axis of
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