Enderlein, Jörg

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10.01.2025

Super-resolution optical fluctuation imaging

Authors Basak S, Chizhik A, Gallea JI, Gligonov I, Gregor I, Nevskyi O, Radmacher N, Tsukanov R, Enderlein J Journal Nature Photonics Citation Nat. Photon. (2025) Abstract We present a comprehensive review of super-resolution optical fluctuation imaging (SOFI), a robust technique that leverages temporal fluctuations in fluorescence intensity to achieve
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18.12.2024

Proton Tunneling Allows a Proton-Coupled Electron Transfer Process in the Cancer Cell

Authors Zhang T, Ghosh A, Behringer-Pließ L, Chouhan L, Cunha AV, Havenith RWA, Butkevich E, Zhang L, Vázquez O, Debroye E, Enderlein J, Das S Journal Journal of the American Chemical Society Citation JACS Au 2024. Abstract Proton-coupled electron transfer (PCET) is a fundamental redox process and has clear advantages
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30.11.2024

Observation of E-cadherin adherens junction dynamics with metal-induced energy transfer imaging and spectroscopy

Authors Chen T, Karedla N, Enderlein J Journal Communications Biology Citation Commun Biol. 2024 Nov 30;7(1):1596. Abstract Epithelial cadherin (E-cad) mediated cell-cell junctions play a crucial role in the establishment and maintenance of tissues and organs. In this study, we employed metal-induced energy transfer imaging and spectroscopy to investigate variations
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27.09.2024

Measuring 3-dimensional dynamics of membrane in living cell with metal-induced energy transfer imaging/spectroscopy

Authors Chen T, Gallea JI, Karedla N, Wang D, Enderlein J Journal BioRxiv Citation bioRxiv 2024.09.27.614973. Abstract The dynamics of cellular membranes are crucial for many essential cellular processes. Despite various methods available to study membrane dynamics, simultaneously measuring membrane diffusion and fluctuations within living cell membranes remains challenging. In
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20.08.2024

Variational calculus approach to Zernike polynomials with application to fluorescence correlation spectroscopy

Authors Gligonov I, Enderlein J Journal Biophysical Journal Citation Biophys J. 2024 Aug 19:S0006-3495(24)00557-5. Abstract Zernike polynomials are a sequence of orthogonal polynomials that play a crucial role in optics and, in particular, modeling microscopy systems. Introduced by Frits Zernike in 1934, they are particularly useful in expressing wavefront aberrations
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02.08.2024

Doubling the resolution of fluorescence-lifetime single-molecule localization microscopy with image scanning microscopy

Authors Radmacher N, Nevskyi O, Gallea JI, Thiele JC, Gregor I, Rizzoli SO, Enderlein J Journal Nature Photonics Citation Nat. Photon. (2024). Abstract In this study, we integrate a single-photon detector array into a confocal laser scanning microscope, enabling the combination of fluorescence-lifetime single-molecule localization microscopy with image scanning microscopy.
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04.04.2024

Three-dimensional multi-target super-resolution microscopy of cells using Metal-Induced Energy Transfer and DNA-PAINT

Authors Oleksiievets N, Mougios N, Jans DC, Hauke L, Thiele JC, Basak S, Jakobs S, Opazo F, Enderlein J, Tsukanov R, Sananbenesi F, Fischer A   Journal BioRxiv   Citation bioRxiv 2024.04.02.587536.   Abstract DNA-points accumulation for imaging in nanoscale topography (DNA-PAINT) is a potent variant of single-molecule localization microscopy
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27.02.2024

Measuring sub-nanometer undulations at microsecond temporal resolution with metal- and graphene-induced energy transfer spectroscopy

Authors Chen T, Karedla N, Enderlein J Journal Nature Communications Citation Nat Commun. 2024 Feb 27;15(1):1789. Abstract Out-of-plane fluctuations, also known as stochastic displacements, of biological membranes play a crucial role in regulating many essential life processes within cells and organelles. Despite the availability of various methods for quantifying membrane
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21.02.2024

Machine learning and advanced statistical analysis for Fluorescence Correlation Spectroscopy

Authors Enderlein J Journal Biophysical Journal Citation Biophys J. 2024 Feb 21:S0006-3495(24)00131-0. Abstract No abstract available. DOI 10.1016/j.bpj.2024.02.011   Pubmed Link
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17.01.2024

Metal-induced energy transfer (MIET) Imaging of cell surface engineering with multivalent DNA nanobrushes

Authors Wang DX, Liu B, Han GM, Kong DM, Enderlein J, Chen T Journal ACS Nano Citation ACS Nano. 2024 Jan 17. Abstract The spacing between cells has a significant impact on cell–cell interactions, which are critical to the fate and function of both individual cells and multicellular organisms. However,
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