Enderlein, Jörg

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20.01.2026

Mapping Optical Chirality with Single Fluorescent Molecules

Authors Marx D, Gligonov I, Malsbenden D, Wöll D, Nevskyi O, Enderlein J. Journal Nano Letters Citation Nano Lett. 2026 Jan 20. Abstract Single fluorescent molecules, acting as ideal point dipoles, offer a unique means to probe light-matter interactions at the nanoscale. Here, we exploit this property to map the
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01.01.2026

Versatile Microfluidics Platform for Enhanced Multitarget Super-Resolution Microscopy

Authors Basak S, Vu KC, Mougios N, Oleksiievets N, Pollack YG, Brandenburg S, Opazo F, Lehnart SE, Enderlein J, Tsukanov R Journal ACS Nano Citation ACS Nano. 2026 Jan 1. Abstract DNA-based Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) is a powerful variant of single-molecule localization microscopy (SMLM) that
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17.12.2025

Self-blinking dye restores efficient use of nanobodies in single-molecule localization microscopy

Authors Basak S, Inamdar K, Pollack YG, Albert L, Jans DC, Jakobs S, Enderlein J, Tsukanov R, Opazo F Journal BioRxiv Citation bioRxiv 2025.12.17.694880 Abstract Direct STORM relies on robust fluorophore blinking; observations suggest that AlexaFluor647 underperforms when coupled to nanobodies, thereby undermining the advantages of nanobodies. We show that
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10.12.2025

Research highlight: new and powerful approach to wide-field fluorescence lifetime imaging with single-molecule sensitivity

Authors Enderlein J Journal eLight Citation eLight 5, 34 (2025). Abstract Historically constrained by the low throughput of traditional Time-Correlated Single-Photon Counting (TCSPC), a new method utilizes an optimized, time-gated single-photon camera acquisition scheme for achieving single-molecule lifetime precision comparable to TCSPC, but across a wide field of view simultaneously.
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24.11.2025

The near-infrared bacteriophytochrome-derived fluorescent protein PENELOPE enables RESOLFT superresolution microscopy

Authors Stumpf D, Jensen N, Mittelheisser C, Keller-Findeisen J, Chizhik AI, Kamper M, Diekmann T, Habenstein F, Jansen I, Enderlein J, Sliwa M, Inamdar K, Hell SW, Jakobs S Journal Proceedings of the National Academy of Sciences of the United States of America Citation Proc Natl Acad Sci U S
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16.10.2025

Nanoscale architecture and dynamics of CaV1.3 channel clusters in cardiac myocytes revealed by single channel nanoscopy

Authors Schwenzer N, Tsukanov R, Kohl T, Basak S, Sobitov I, Seibertz F, Kapoor R, Voigt N, Enderlein J, Lehnart SE   Journal Journal of Molecular and Cellular Cardiology Plus   Citation J Mol Cell Cardiol Plus. 2025 Oct 16;14:100490.   Abstract The clustering of L-type calcium channels (LTCC) for
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07.10.2025

Mapping complex optical light field distributions with single fluorescent molecules

Authors Marx D, Gligonov I, Malsbenden D, Wöll D, Nevskyi O, Enderlein J Journal Arxiv Citation arXiv:2510.05791. Abstract Single fluorescent molecules, behaving as ideal electric dipole emitters, are powerful nanoscopic probes of complex optical fields. Here, this property is exploited to precisely map the polarization and vectorial structure of tightly
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11.09.2025

Charge-Dependent Fluorescence Lifetime Modulation in a Plasmonic Nanocavity

Authors Chizhik AI, Sakhapov DI, Gregor I, Karedla N, Enderlein J Journal The journal of Physical Chemistry Letters Citation J Phys Chem Lett. 2025 Sep 11:9900-9905. Abstract Electric charges play a fundamental role in shaping the structure, function, and interactions of biomolecules, yet precisely measuring these charges at the single-molecule
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11.09.2025

Charge-Dependent Fluorescence Lifetime Modulation in a Plasmonic Nanocavity

Authors Chizhik AI, Sakhapov DI, Gregor I, Karedla N, Enderlein J Journal The Journal of Physical Chemistry Letters Citation Phys Chem Lett. 2025 Sep 11:9900-9905. Abstract Electric charges play a fundamental role in shaping the structure, function, and interactions of biomolecules, yet precisely measuring these charges at the single-molecule level
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18.08.2025

Super-Resolution Axial Imaging for Quantifying Piconewton Traction Forces in Live Cells

Authors Wang DX, Gallea JI, Kong DM, Enderlein J, Chen T Journal Angewandte Chemie (International ed. in Engl.) Citation Angew Chem Int Ed Engl. 2025 Aug 18:e202506864. Abstract Cell mechanics play a pivotal role in regulating numerous biological processes. Although super-resolution microscopy enables the imaging of cellular forces in the
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