Advanced fluorescence correlation spectroscopy for studying biomolecular conformation

Authors

Ghosh A, Enderlein J

Journal

Current Opinion in Structural Biology

Citation

Curr Opin Struct Biol. 2021 Aug 6;70:123-131.

Abstract

We present the recent developments and advances in fluorescence correlation spectroscopy (FCS) and their application to the investigation of biomolecular conformations. In particular, we present and discuss three techniques: multichannel nanosecond FCS, photo-induced electron transfer FCS, and fluorescence lifetime correlation spectroscopy. We briefly describe each method and discuss recent applications to diverse biophysical studies of biomolecular conformation.

DOI

10.1016/j.sbi.2021.06.018
 

Pubmed Link