Ruhlandt D, Katti A, Derouard J, Delon A, Enderlein J
Fluorescence Correlation Spectroscopy (FCS) is a powerful single-molecule technique which allows for measuring motion (diffusion, flow), concentration, and molecular interaction kinetics of fluorescent molecules from picomolar to micromolar concentrations. It has found manifold applications in the physical and life sciences. Many biological/biophysical applications use FCS for measuring the motion and concentration of fluorescently labeled biomolecules in living cells and tissue. However, a correct quantitative evaluation of FCS experiments relies on the accurate knowledge of the fluorescence excitation and detection properties of the used confocal microscope. Using a bottom-up approach, we theoretically study how these properties are affected by the presence of a diffracting dielectric bead within the optical path, and how this changes the outcome of a FCS measurement. This will be important for all applications of FCS under optically non-ideal aberrating conditions.
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