Lehnart, Stephan

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24.10.2024

Unbiased complexome profiling and global proteomics analysis reveals mitochondrial impairment and potential changes at the intercalated disk in presymptomatic R14Δ/+ mice hearts

Authors Foo B, Amedei H, Kaur S, Jaawan S, Boshnakovska A, Gall T, de Boer RA, Silljé HHW, Urlaub H, Rehling P, Lenz C, Lehnart SE Journal PLoS One Citation PLoS One. 2024 Oct 24;19(10):e0311203. Abstract Phospholamban (PLN) is a sarco-endoplasmic reticulum (SER) membrane protein that regulates cardiac contraction/relaxation by
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01.08.2024

mini-Complexome Profiling (mCP), an FDR-controlled workflow for global targeted detection of protein complexes

Authors Amedei H, Paul NB, Foo B, Neuenroth L, Lehnart SE, Urlaub H, Lenz C Journal BioRxiv Citation Front. Anal. Sci. 4:1425190. Abstract Introduction: Co-fractionation mass spectrometry couples native-like separations of protein-protein complexes with mass spectrometric proteome analysis for global characterization of protein networks. The technique allows for both de
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16.07.2024

Dysferlin Enables Tubular Membrane Proliferation in Cardiac Hypertrophy

Authors Paulke NJ, Fleischhacker C, Wegener JB, Riedemann GC, Cretu C, Mushtaq M, Zaremba N, Möbius W, Zühlke Y, Wedemeyer J, Liebmann L, Gorshkova AA, Kownatzki-Danger D, Wagner E, Kohl T, Wichmann C, Jahn O, Urlaub H, Toischer K, Hasenfuß G, Moser T, Preobraschenski J, Lenz C, Rog-Zielinska EA, Lehnart
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25.06.2024

Structural insights into the regulation of RyR1 by S100A1

Authors Weninger G, Miotto MC, Tchagou C, Reiken S, Dridi H, Brandenburg S, Riedemann GC, Yuan Q, Liu Y, Chang A, Wronska A, Lehnart SE, Marks AR Journal Proceedings of the National Academy of Sciences of the United States of America Citation Proc Natl Acad Sci U S A. 2024
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24.06.2024

Impaired Intracellular Calcium Buffering Contributes to the Arrhythmogenic Substrate in Atrial Myocytes From Patients With Atrial Fibrillation

Authors Fakuade FE, Hubricht D, Möller V, Sobitov I, Liutkute A, Döring Y, Seibertz F, Gerloff M, Pronto JRD, Haghighi F, Brandenburg S, Alhussini K, Ignatyeva N, Bonhoff Y, Kestel S, El-Essawi A, Jebran AF, Großmann M, Danner BC, Baraki H, Schmidt C, Sossalla S, Kutschka I, Bening C, Maack
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07.06.2024

Establishing synthetic ribbon-type active zones in a heterologous expression system

Authors Kapoor R, Schwenzer N, Dresbach T, Lehnart S, Moser T Journal eLife Citation eLife 13:RP98254. Abstract Encoding of several sensory modalities into neural signals is mediated by ribbon synapses. The synaptic ribbon tethers synaptic vesicles at the presynaptic active zone (AZ) and might act as a super-scaffold organizing AZ
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01.06.2024

Analyzing cross-talk between superimposed signals: Vector norm dependent hidden Markov models and applications to ion channels

Authors Vanegas LJ, Eltzner B, Rudolf D, Dura M, Lehnart SE, Munk A Journal The Annals of Applied Statistics Citation Ann. Appl. Stat. 18(2): 1445-1470. Abstract We propose and investigate a hidden Markov model (HMM) for the analysis of dependent, aggregated, superimposed two-state signal recordings. A major motivation for this
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23.05.2024

CaV1.3 channel clusters characterized by live-cell and isolated plasma membrane nanoscopy

Authors Schwenzer N, Teiwes NK, Kohl T, Pohl C, Giller MJ, Lehnart SE, Steinem C Journal Communications Biology Citation Commun Biol. 2024 May 23;7(1):620. Abstract A key player of excitable cells in the heart and brain is the L-type calcium channel CaV1.3. In the heart, it is required for voltage-dependent
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10.03.2024

3D Super-Resolution Nuclear Q-FISH Imaging Reveals Cell-Cycle-Related Telomere Changes

Authors Pochechueva TV, Schwenzer N, Kohl T, Brandenburg S, Kaltenecker G, Wollnik B, Lehnart SE Journal International Journal of Molecular Sciences Citation Int J Mol Sci. 2024 Mar 10;25(6):3183. Abstract We present novel workflows for Q-FISH nanoscopy with the potential for prognostic applications and resolving novel chromatin compaction changes. DNA-fluorescence
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28.02.2024

Nanoscale architecture and dynamics of CaV1.3 channel clusters in cardiac myocytes revealed by single channel nanoscopy

Authors Schwenzer N, Tsukanov R, Kohl T, Basak S, Seibertz F, Voigt N, Enderlein J, Lehnart SE   Journal BioRxiv   Citation bioRxiv 2024.02.26.582084.   Abstract The clustering of L-type calcium channels in cardiac myocytes presents an important mechanism for functional regulation of calcium signaling. Here we applied targeted super-resolution
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