Hell, Stefan

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13.04.2023

A Bright Surprise: Live-Cell Labeling with Negatively Charged Fluorescent Probes based on Disulfonated Rhodamines and HaloTag

Authors Kim D, Stoldt S, Weber M, Jakobs S, Belov VN, Hell SW   Journal Chemistry Methods   Citation Chem. Methods 2023, e202200076.   Abstract Disulfonated rhodamines are photostable and bright dyes widely used in life science and optical microscopy. However, di-sulfonated dyes were considered cell impermeable and not applicable
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01.09.2022

DNA-PAINT MINFLUX nanoscopy

Authors Ostersehlt LM, Jans DC, Wittek A, Keller-Findeisen J, Inamdar K, Sahl SJ, Hell SW, Jakobs S Journal Nature Methods Citation Nat Methods (2022). Abstract MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations, has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on–off switching
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08.03.2022

Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3

Authors Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, Urlaub H, Riedel D, Preobraschenski J, Milosevic I, Hell SW, Jahn R, Sambandan S Journal Neuron Citation Neuron. 2022 Mar 4:S0896-6273(22)00146-5. Abstract Vesicular transporters (VTs) define the
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15.03.2021

MINSTED fluorescence localization and nanoscopy

Authors Weber M, Leutenegger M, Stoldt S, Jakobs S, Mihaila TS, Butkevich AN, Hell SW Journal Nature Photonics Citation Nat. Photonics 15, 361–366 (2021). Abstract We introduce MINSTED, a fluorophore localization and super-resolution microscopy concept based on stimulated emission depletion (STED) that provides spatial precision and resolution down to the
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11.08.2020

Multicolor 3D MINFLUX nanoscopy of mitochondrial MICOS proteins

Authors Pape JK, Stephan T, Balzarotti F, Büchner R, Lange F, Riedel D, Jakobs S, Hell SW Journal Proceedings of the National Academy of Sciences Citation PNAS, Aug 2020, 202009364. Abstract The mitochondrial contact site and cristae organizing system (MICOS) is a multisubunit protein complex that is essential for the
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22.06.2020

MICOS assembly controls mitochondrial inner membrane remodeling and crista junction redistribution to mediate cristae formation

Authors Stephan T, Bruser C, Deckers M, Steyer M, Balzarotti F, Barbot M, Behr TS, Heim G, Hubner W, Ilgen P, Lange F, Pacheu-Grau D, Pape JK, Stoldt S, Huser T, Hell SW, Möbius W, Rehling P, Riedel D, Jakobs S Journal EMBO Journal Citation EMBO J 2020 Jun 22;e104105
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22.07.2019

Molecular contribution function in RESOLFT nanoscopy

Authors Frahm L, Keller-Findeisen J, Alt P, Schnorrenberg S, Del Alamo Ruiz M, Aspelmeier T, Munk A, Jakobs S, Hell SW Journal Optics Express Citation Opt Express. 2019 Jul 22;27(15):21956-21987. Abstract BACKGROUND: The ultimate objective of a microscope of the highest resolution is to map the molecules of interest in
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