Cramer, Patrick

Authors Quililan K, Oberbeckmann E, Cramer P, Oudelaar AM   Journal BioRxiv   Citation bioRxiv 2023.02.27.530214.   Abstract The spatial organization of the genome modulates nuclear processes, including transcription, replication, and DNA repair1,2. Eukaryotic genomes are organized into distinct 3D chromatin domains3. However, the molecular mechanisms that drive the formation

Authors Rengachari S, Schilbach S, Kaliyappan T, Gouge J, Zumer K, Schwarz J, Urlaub H, Dienemann C, Vannini A, Cramer P Journal Nature Structural and Molecular Biology Citation Nat Struct Mol Biol. 2022 Nov 24. Abstract RNA polymerase II (Pol II) carries out transcription of both protein-coding and non-coding genes.

Authors Wang H, Schilbach S, Ninov M, Urlaub H, Cramer P Journal Nature Structural & Molecular Biology Citation Nat Struct Mol Biol. 2022 Nov 21. Abstract The preinitiation complex (PIC) assembles on promoters of protein-coding genes to position RNA polymerase II (Pol II) for transcription initiation. Previous structural studies revealed

Authors Farnung L, Ochmann M, Garg G, Vos SM, Cramer P Journal Molecular Cell Citation Mol Cell. 2022 Jul 13:S1097-2765(22)00610-4. Abstract During gene transcription, RNA polymerase II (RNA Pol II) passes nucleosomes with the help of various elongation factors. Here, we show that RNA Pol II achieves efficient nucleosome passage

Authors Xiong L, Tolen EA, Choi J, Velychko S, Caizzi L, Velychko T, Adachi K, MacCarthy CM, Lidschreiber M, Cramer P, Schoeler HR Journal eLife Citation eLife 11:e71533. Abstract The transcription factor Oct4 is essential for the maintenance and induction of stem cell pluripotency, but its functional roles are not

Authors Dombrowski M, Engeholm M, Dienemann C, Dodonova, S. Cramer P Journal Nature Structural & Molecular Biology Citation Nat Struct Mol Biol 29, 493–501 (2022). Abstract Throughout the genome, nucleosomes often form regular arrays that differ in nucleosome repeat length (NRL), occupancy of linker histone H1 and transcriptional activity. Here,

Authors Fianu I, Chen Y, Dienemann Ch, Dybkov O, Linden A, Urlaub H, Cramer P Journal Science Citation Science. 2021 Nov 12;374(6569):883-887 Abstract Integrator and protein phosphatase 2A (PP2A) form a complex that dephosphorylates paused RNA polymerase II (Pol II), cleaves the nascent RNA, and terminates transcription. We report the

Authors Aibara S, Dienemann Ch, Cramer P Journal Nucleic Acids Research Citation Nucleic Acids Research, 2021; Sep 16, gkab783 Abstract Eukaryotic gene transcription is carried out by three RNA polymerases: Pol I, Pol II and Pol III. Although it has long been known that Pol I can form homodimers, it

Authors Kokic G, Wagner FR, Chernev A, Urlaub H, Cramer P Journal Nature Citation Nature 2021 Abstract Transcription-coupled DNA repair removes bulky DNA lesions from the genome and protects cells against ultraviolet (UV) irradiation. Transcription-coupled DNA repair begins when RNA polymerase II (Pol II) stalls at a DNA lesion and recruits

Authors Bhatta A, Dienemann C, Cramer P, Hillen HS Journal Nature Structural and Molecular Biology Citation Nat Struct Mol Biol (2021). Abstract Human mitochondrial transcripts contain messenger and ribosomal RNAs flanked by transfer RNAs (tRNAs), which are excised by mitochondrial RNase (mtRNase) P and Z to liberate all RNA species.