Egner, Alexander

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15.04.2024

Click Chemistry with Cell-Permeable Fluorophores Expands the Choice of Bioorthogonal Markers for Two-Color Live-Cell STED Nanoscopy

Authors Gregor C, Grimm F, Rehman J, Wurm CA, Egner A Journal Cells Citation Cells 2024, 13(8), 683. Abstract STED nanoscopy allows for the direct observation of dynamic processes in living cells and tissues with diffraction-unlimited resolution. Although fluorescent proteins can be used for STED imaging, these labels are often
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23.01.2023

Towards Unbiased Fluorophore Counting in Superresolution Fluorescence Microscopy

Authors Laitenberger O, Aspelmeier T, Staudt T, Geisler C, Munk A, Egner A Journal Nanomaterials Citation Nanomaterials 2023, 13(3), 459. Abstract With the advent of fluorescence superresolution microscopy, nano-sized structures can be imaged with a previously unprecedented accuracy. Therefore, it is rapidly gaining importance as an analytical tool in the
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09.07.2021

isoSTED microscopy with water-immersion lenses and background reduction

Authors Siegmund R, Werner F, Jakobs S, Geisler C, Egner A Journal Biophysical Journal Citation Biophys J. 2021 Jul 8:S0006-3495(21)00556-7. Abstract Fluorescence microscopy is an excellent tool to gain knowledge on cellular structures and biochemical processes. Stimulated emission depletion (STED) microscopy provides a resolution in the range of a few
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19.05.2020

Tomographic STED microscopy

Authors Krüger JR, Keller-Findeisen J, Geisler C, Egner A Journal Biomedical Optics Express Citation Biomed. Opt. Express 11, 3139-3163 (2020). Abstract Stimulated emission depletion (STED) microscopy is a versatile imaging method with diffraction-unlimited resolution. Here, we present a novel STED microscopy variant that achieves either increased resolution at equal laser
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03.03.2020

Statistical Molecule Counting in Super-Resolution Fluorescence Microscopy: Towards Quantitative Nanoscopy

Authors Staudt T, Aspelmeier T, Laitenberger O, Geisler C, Egner A, Munk A Journal Statistical Science Citation Statist. Sci., Volume 35, Number 1 (2020), 92-111. Abstract Super-resolution microscopy is rapidly gaining importance as an analytical tool in the life sciences. A compelling feature is the ability to label biological units
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17.02.2020

Pixel hopping enables fast STED nanoscopy at low light dose

Authors Vinçon B, Geisler C, Egner A Journal Optics Express Citation Opt. Express 28, 4516-4528 (2020). Abstract The achievable image quality in fluorescence microscopy and nanoscopy is usually limited by photobleaching. Reducing the light dose imposed on the sample is thus a challenge for all these imaging techniques. Various approaches
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