Authors
Kloskowski P, Neumann P, Berndt A, Ficner R
Journal
Acta Crystallographica Section F-Structural Biology Communications
Citation
Acta Crystallogr F Struct Biol Commun. 2025 Aug 1.
Abstract
Nucleotide-bound crystal structures of SARS-CoV-2 NSP13 in ADP- and ATP-bound states were resolved to 1.8 and 1.9 Å, respectively. The ADP-bound model captures a state immediately following ATP hydrolysis, with both ADP and orthophosphate still present in the active site. Further comparative analysis revealed that crystal packing influences NSP13 by stabilizing the nucleotide-binding site, underscoring the importance of accounting for these effects in structure-based drug design targeting NSP13.
