2024

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02.08.2024

Doubling the resolution of fluorescence-lifetime single-molecule localization microscopy with image scanning microscopy

Authors Radmacher N, Nevskyi O, Gallea JI, Thiele JC, Gregor I, Rizzoli SO, Enderlein J Journal Nature Photonics Citation Nat. Photon. (2024). Abstract In this study, we integrate a single-photon detector array into a confocal laser scanning microscope, enabling the combination of fluorescence-lifetime single-molecule localization microscopy with image scanning microscopy.
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01.08.2024

mini-Complexome Profiling (mCP), an FDR-controlled workflow for global targeted detection of protein complexes

Authors Amedei H, Paul NB, Foo B, Neuenroth L, Lehnart SE, Urlaub H, Lenz C Journal BioRxiv Citation Front. Anal. Sci. 4:1425190. Abstract Introduction: Co-fractionation mass spectrometry couples native-like separations of protein-protein complexes with mass spectrometric proteome analysis for global characterization of protein networks. The technique allows for both de
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Blick in die Zelle per optische Laserfalle: Sie lokalisiert mikroskopische Teilchen, um auf ihre zufällige Zitterbewegung rückzuschließen. Ein neuer Ansatz der Göttinger Forschenden erlaubt es aus diesen Bewegungen zu schließen, wie hart, weich oder flüssig das Zellinnere ist. Foto: Till Moritz Münker

31.07.2024

„Laserblick“ in die Avocado: Neues Verfahren enthüllt Zellinneres

Forscherteam der Universität Göttingen entwickelt Methode zum Erkennen von Zelleigenschaften   Durch Hinsehen prüfen, ob eine Avocado hart oder weich ist? Dazu müsste man erkennen, wie sich die Pflanzenzellen hinter der Schale verhalten. Gleiches gilt für alle anderen Zellen auf unserem Planeten: Trotz mehr als 100 Jahren intensiver Forschung bleiben
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31.07.2024

Accessing activity and viscoelastic properties of artificial and living systems from passive measurement

Authors Muenker TM, Knotz G, Krüger M, Betz T Journal Nature Materials Citation Nat Mater. 2024 Jul 31. Abstract Living systems are complex dynamic entities that operate far from thermodynamic equilibrium. Their active, non-equilibrium behaviour requires energy to drive cellular organization and dynamics. Unfortunately, most statistical mechanics approaches are not
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28.07.2024

Helical superstructures between amyloid and collagen VI in heart-derived fibrils from a patient with Light Chain Amyloidosis

Authors Schulte T, Chaves-Sanjuan A, Speranzini V, Sicking K, Milazzo M, Mazzini G, Rognoni P, Caminito S, Milani P, Marabelli C, Corbelli A, Diomede L, Fiordaliso F, Anastasia L, Pappone C, Merlini G, Bolognesi M, Nuvolone M, Fernández-Busnadiego R, Palladini G, Ricagno S Journal Nature Communications Citation Nat Commun. 2024
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24.07.2024

Microlens arrays for multichannel laser-to-waveguide coupling

Authors Kunze K, Gossler C, Peters V, Keppeler D, Moser T, Schwarz UT Journal Applied Optics Citation Appl. Opt. 63, 5876-5885 (2024). Abstract An optical multichannel coupling system for coupling laser arrays to waveguide arrays is developed. Based on a microlens array, the system enables coupling of nine individual optical
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22.07.2024

Transient Non-local Interactions Dominate the Dynamics of Measles Virus NTAIL

Authors Otteson L, Nagy G, Kunkel J, Kodis G, Zheng W, Bignon C, Longhi S, Grubmüller H, Vaiana AC, Vaiana SM Journal BioRxiv Citation bioRxiv 2024.07.22.604679. Abstract The RNA genome of measles virus is encapsidated by the nucleoprotein within a helical nucleocapsid that serves as template for both transcription and
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21.07.2024

Distributional limits of graph cuts on discretized grids

Authors Suchan L, Li H, Munk A Journal Arxiv Citation arXiv:2407.15297. Abstract Graph cuts are among the most prominent tools for clustering and classification analysis. While intensively studied from geometric and algorithmic perspectives, graph cut-based statistical inference still remains elusive to a certain extent. Distributional limits are fundamental in understanding
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19.07.2024

Peripheral whole blood microRNA expression in relation to vascular function: a population-based study

Authors Talevi V, Melas K, Pehlivan G, Imtiaz MA, Krüger DM, Centeno TP, Aziz NA, Fischer A, Breteler MMB Journal Journal of Translational Medicine Citation J Transl Med. 2024 Jul 19;22(1):670. Abstract Background: As key regulators of gene expression, microRNAs affect many cardiovascular mechanisms and have been associated with several
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18.07.2024

FASTKD5 processes mitochondrial pre-mRNAs at non-canonical cleavage sites

Authors Antonicka H, Weraarpachai W, Vučković A, Hillen HS, Shoubridge EA Journal BioRxiv Citation bioRxiv 2024.07.18.603998. Abstract The regulation of mammalian mitochondrial gene expression is largely post-transcriptional and the first step in translating the 13 polypeptides encoded in mtDNA is endonucleolytic cleavage of the primary polycistronic transcripts. As the rRNAs
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