Three-dimensional virtual histology of the cerebral cortex based on phase-contrast X-ray tomography


Eckermann M, van der Meer F, Cloetens P, Ruhwedel T, Möbius W, Stadelmann C, Salditt T


Biomedical Optics Express


Biomed. Opt. Express 12, 7582-7598 (2021).


In this work, we optimize the setups and experimental parameters of X-ray phase-contrast computed-tomography for the three-dimensional imaging of the cyto- and myeloarchitecture of cerebral cortex, including both human and murine tissue. We present examples for different optical configurations using state-of-the art synchrotron instruments for holographic tomography, as well as compact laboratory setups for phase-contrast tomography in the direct contrast (edge-enhancement) regime. Apart from unstained and paraffin-embedded tissue, we tested hydrated tissue, as well as heavy metal stained and resin-embedded tissue using two different protocols. Further, we show that the image quality achieved allows to assess the neuropathology of multiple sclerosis in a biopsy sample collected during surgery.



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