Generation of a fluorescent oligodendrocyte reporter line in human induced pluripotent stem cells


Schreiber MK, Zafeiriou MP


Stem Cell Research


Stem Cell Res, 21 December 2023, 103295.


Human brain organoids can serve as models to study myelination, a process orchestrated by oligodendrocytes. Real-time imaging provides new insights on the communication of oligodendrocytes with neurons as well as demyelination processes in patient derived organoids. PLP1, a prominent myelin protein within the central nervous system, is associated with demyelinating diseases, such as Pelizaeus-Merzbacher. In this study, we generated a stable PLP1-Citrine reporter line (fPLP1) in human induced pluripotent stem cells (iPSCs) by CRISPR/Cas9 editing. fPLP1 facilitates visualization of PLP1 expression in living brain organoids, allowing time-lapse imaging of pre-myelinating and myelinating oligodendrocytes.


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