Microscopy Club Lecture

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june, 2024

202427jun1:00 PM2:00 PMMicroscopy Club LectureEnhanced contrast mechanisms for live cell structured illumination microscopy1:00 PM - 2:00 PM Institut für Nanophotonik (IFNANO), Hans-Adolf-Krebs Weg 1, 37077 GöttingenSpeaker:Prof. Dr. Thomas Huser, University of Bielefeld

Event Details

Host: Jörg Enderlein (U GOE), MBExC
Local organizer: Alexander Egner (IFNANO)

Speaker: Prof. Dr. Thomas Huser, Department of Physics, University of Bielefeld

Title: Enhanced contrast mechanisms for live cell structured illumination microscopy

Super-resolved structured illumination microscopy (SR-SIM) is a widely used fluorescence microscopy technique capable of surpassing the optical diffraction limit, which employs rapid image acquisition, imaging at multiple color channels, and true 3D imaging capability. The basic optical physics behind structured illumination does, however, promise a much wider range of potential contrast mechanisms. We have recently developed a new generation of compact, cost-efficient and high-speed SR-SIM modalities based on a fiber-optic implementation that takes advantage of novel sCMOS cameras with up to 26 MegaPixel. This enabled us to obtain a significantly increased field-of-view of up to 280 µm x 280 µm with video-rate image acquisition, while still providing 100 nm spatial resolution. By combining this with a unique prism-based telescope, we can now also seamlessly transition from 2D/3D SIM to grazing incidence illumination and TIRF-SIM with different TIRF angles. This opens up a pathway to increased axial resolution. Furthermore, by combining this modality with a fast electrooptic gate, SIM with fluorescence lifetime imaging becomes possible. I will discuss novel applications of these enhanced contrast mechanisms in live cell imaging. In particular, I will demonstrate how we utilize this to provide new insights into the fast dynamics of liver sinusoidal endothelial cells during their scavenging activity.

Please find here the PDF of the announcement: