Microscopy Club Lecture

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june, 2022

202230jun1:00 PM2:00 PMVirtual EventMicroscopy Club LecturePushing the boundaries of cryo-EM for GPCRs1:00 PM - 2:00 PM OnlineSpeaker:Radostin Danev, Ph.D., Graduate School of Medicine, The University of Tokyo, Japan

Event Details

Chair: Eri Sakata, UMG/MBExC

Speaker: Radostin Danev, Ph.D., Graduate School of Medicine, The University of Tokyo, Japan

Pushing the boundaries of cryo-EM for GPCRs

Abstract: Cryo-electron microscopy (cryo-EM) continues to grow as a powerful method for structural studies of biomolecules and their complexes. Nowadays, it can routinely determine molecular structures with resolutions in the 2.5 – 3.5 Å range. Such results are adequate for modelling of the protein but lack fidelity for confident localization of water molecules and hydrogen atoms. Unambiguous elucidation of the biochemistry behind protein function and pharmacology of drugs would require atomic resolution structures, at levels below 1.5 Å. Last year, several groups worldwide demonstrated atomic resolution cryo-EM with a test sample comprising the “easy” soluble protein apoferritin. This was an important technological milestone showcasing the best-case-scenario capabilities of cryo-EM. However, membrane proteins, and other real-world samples, impose numerous experimental challenges, such as small size, heterogeneity, flexibility, preferential orientation, etc. The talk will be about optimizing the performance of cryo-EM for challenging membrane proteins, and particularly in studies of the structure, pharmacology, and dynamics of G protein-coupled receptors.

Host: Carola Gregor (MBExC/IFNANO), Jan Huisken (U Göttingen), Eri Sakata (UMG)

Local organizers: Alexander Egner (IFNANO), Jörg Enderlein (U Göttingen)