Event Type Optogenetics Club Lecture
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Optogenetics Club Lecture
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event speaker
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Tobias Moser
Vladan Rankovic
Rubén Fernández-Busnadiego
Peter Rehling
Antonio Martinez-Sanchez
Han Chen
Hauke Hillen
Housen Li
Julia Preobraschenski
Jörg Wegener
Ricarda Richter-Dennerlein
Sarah Köster
Stefan Stoldt
Thomas Oertner
Tiago Outeiro
Tim Salditt
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Cell biology
Cryo-electron tomography
Cell biology
Cryo-electron tomography
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august
Event Details
Prof. Dr. Volker Busskamp from the Department of Ophthalmology, University of Bonn will talk about “Driving human stem-cell derived neuronal circuits by optogenetics” Abstract The advent of human induced pluripotent stem cells
Event Details
Prof. Dr. Volker Busskamp from the Department of Ophthalmology, University of Bonn will talk about “Driving human stem-cell derived neuronal circuits by optogenetics”
Abstract
The advent of human induced pluripotent stem cells (hiPSCs) now facilitates bottom-up approaches in neuroscience. There exist many efficient protocols to trigger neurogenesis in hiPSCs. We are using known and newly-screened transcription factors to forward program hiPSCs into neurons. We study their functional maturation over time using standard and CMOS-micro-electrode array (MEA) recordings, enabling continuous assessment of neuronal function over three months. We show that individual neurons, expressing optogenes, are efficiently stimulated using holographic stimulation. This technique also facilitates to study individual neuronal circuits in random networks in 2D. Optogenetics is beneficial to induce robust neuronal activity at earlier developmental time points. Next to random networks, we also developed a “cell-jet” printer to generate precise and reproducible neuronal circuits in microchannels placed on MEAs. Thereby, we study circuit development and functional features of individual neurons.
Chairs: Tobias Moser and Thomas Mager
Organizer
MBExC