Targeting of Membrane Proteins

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16.06.2023

Diversity of Ganglion Cell Responses to Saccade-like Image Shifts in the Primate Retina

Authors Krüppel S, Khani MH, Karamanlis D, Erol YC, Zapp SJ, Mietsch M, Protti DA, Rozenblit F, Gollisch T Journal Journal of Neuroscience Citation J Neurosci. 2023 Jun 16:JN-RM-1561-22. Abstract Saccades are a fundamental part of natural vision. They interrupt fixations of the visual gaze and rapidly shift the image
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02.02.2023

The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds in a cellular system

Authors Saha I, Yuste-Checa P, Da Silva Padilha M, Guo Q, Körner R, Holthusen H, Trinkaus VA, Dudanova I, Fernández-Busnadiego R, Baumeister W, Sanders DW, Gautam S, Diamond MI, Hartl FU, Hipp MS Journal Nature Communications Citation Nat Commun. 2023 Feb 2;14(1):560. Abstract Amyloid-like aggregates of the microtubule-associated protein Tau
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12.12.2022

Mono- and Intralink Filter (Mi-Filter) To Reduce False Identifications in Cross-Linking Mass Spectrometry Data

Authors Chen X, Sailer C, Kammer KM, Fürsch J, Eisele MR, Sakata E, Pellarin R, Stengel F Journal Analytical Chemistry Citation Anal. Chem. 2022, 94, 51, 17751–17756. Abstract Cross-linking mass spectrometry (XL-MS) has become an indispensable tool for the emerging field of systems structural biology over the recent years. However,
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06.07.2022

Single-Molecule Fluorescence Lifetime Imaging Using Wide-Field and Confocal-Laser Scanning Microscopy: A Comparative Analysis

Authors Oleksiievets N, Mathew C, Thiele JC, Gallea J, Nevskyi O, Gregor I, Weber A, Tsukanov R, Enderlein J Journal Nano Letters Citation Nano Lett. 2022. Abstract A recent addition to the toolbox of super-resolution microscopy methods is fluorescence-lifetime single-molecule localization microscopy (FL-SMLM). The synergy of SMLM and fluorescence-lifetime imaging
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21.04.2022

A Reliable Approach for Revealing Molecular Targets in Secondary Ion Mass Spectrometry

Authors Li F, Fornasiero EF, Dankovich TM, Kluever V, Rizzoli SO Journal International Journal of Molecular Sciences Citation Int. J. Mol. Sci. 2022, 23, 4615. Abstract Nano secondary ion mass spectrometry (nanoSIMS) imaging is a rapidly growing field in biological sciences, which enables investigators to describe the chemical composition of
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21.12.2021

Amyloid-like aggregates cause lysosomal defects in neurons via gain-of-function toxicity

Authors Riera-Tur I, Schäfer T, Hornburg D, Mishra A, da Silva Padilha M, Fernández-Mosquera L, Feigenbutz D, Auer P, Mann M, Baumeister W, Klein R, Meissner F, Raimundo N, Fernández-Busnadiego R, Dudanova I Journal Life Science Alliance Citation Life Sci Alliance. 2021 Dec 21;5(3):e202101185. Abstract The autophagy-lysosomal pathway is impaired
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15.12.2021

Super-resolution imaging: when biophysics meets nanophotonics

Authors Koenderink AF, Tsukanov R, Enderlein J, Izeddin I, Krachmalnicoff V Journal Nanophotonics Citation Nanophotonics, vol. 11, no. 2, 2022, pp. 169-202. Abstract Probing light–matter interaction at the nanometer scale is one of the most fascinating topics of modern optics. Its importance is underlined by the large span of fields
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25.10.2021

Cnm1 mediates nucleus-mitochondria contact site formation in response to phospholipid levels

Authors Eisenberg-Bord M, Zung N, Collado J, Drwesh L, Fenech EJ, Fadel A, Dezorella N, Bykov YS, Rapaport D, Fernandez-Busnadiego R, Schuldiner M Journal Journal of Cell Biology Citation J Cell Biol. 2021 Nov 1;220(11):e202104100. Abstract Mitochondrial functions are tightly regulated by nuclear activity, requiring extensive communication between these organelles.
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17.06.2021

Concurrent axon and myelin destruction differentiates X-linked adrenoleukodystrophy from multiple sclerosis

Authors Bergner CG, Genc N, Hametner S, Franz J, van der Meer F, Mitkovski M, Weber MS, Stoltenburg-Didinger G, Kühl JS, Köhler W, Brück W, Gärtner J, Stadelmann-Nessler C Journal Glia Citation Glia, 2021, 1-16. Abstract Cerebral disease manifestation occurs in about two thirds of males with X-linked adrenoleukodystrophy (CALD)
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16.06.2021

Mapping Activity-Dependent Quasi-Stationary States of Mitochondrial Membranes with Graphene-Induced Energy Transfer Imaging

Authors Raja SO, Chizhik AI, Schmidt CF, Enderlein J, Ghosh A Journal Nano Letters Citation Nano Lett. 2021, 21, 8244−8249 Abstract Graphene-induced energy transfer (GIET) was recently introduced for sub-nanometric axial localization of fluorescent molecules. GIET relies on near-field energy transfer from an optically excited fluorophore to a single sheet
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