Stem Cell Research

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26.08.2021

Establishment of a second generation homozygous CRISPRa human induced pluripotent stem cell (hiPSC) line for enhanced levels of endogenous gene activation

Authors Schoger E, Zimmermann WH, Cyganek L, Zelarayán LC Journal Stem Cell Research Citation Stem Cell Res. 2021 Aug 26;56:102518. Abstract CRISPR/Cas9 technology based on nuclease inactive dCas9 and fused to the heterotrimeric VPR transcriptional activator is a powerful tool to enhance endogenous transcription by targeting defined genomic loci. We
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27.07.2021

Establishment of two homozygous CRISPR interference (CRISPRi) knock-in human induced pluripotent stem cell (hiPSC) lines for titratable endogenous gene repression

Authors Schoger E, Zimmermann WH, Cyganek L, Zelarayán LC Journal Stem Cell Research Citation Stem Cell Res. 2021 Jul 27;55:102473. Abstract Using nuclease-deficient dead (d)Cas9 without enzymatic activity fused to transcriptional inhibitors (CRISPRi) allows for transcriptional interference and results in a powerful tool for the elucidation of developmental, homeostatic and
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