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A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming

Authors Lyra-Leite DM, Gutiérrez-Gutiérrez Ó, Wang M, Zhou Y, Cyganek L, Burridge PW Journal Star Protocols Citation STAR Protoc. 2022 Aug 18;3(3):101560. Abstract The methods for the culture and cardiomyocyte differentiation of human embryonic stem cells, and later human induced pluripotent stem cells (hiPSC), have moved from a complex and
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Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue

Authors Sakib MS, Sokpor G, Nguyen HP, Fischer A, Tuoc T Journal Star Protocols Citation STAR Protoc. 2021 Feb 2;2(1):100318. Abstract Cell sorting can be used to purify cell populations for cell type-specific molecular probing. Fluorescence-activated cell sorting (FACS) coupled with high-throughput sequencing affords molecular signature identification for specific cell
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Generation of Engineered Human Myocardium in a Multi-well Format

Authors Tiburcy M, Meyer T, Liaw NY, Zimmermann WH Journal STAR protocols Citation STAR Protoc. 2020 Jun 3;1(1):100032. Abstract This protocol describes a robust method for the generation of engineered human myocardium (EHM) from pluripotent stem cells (PSCs) in a multi-well plate under defined, serum-free conditions. By parallel culture of
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