STAR protocols

Authors Shahriyari M, Rinn M, Hofemeier AD, Babych A, Zimmermann WH, Tiburcy M Journal Star Protocols Citation STAR Protoc. 2024 Mar 15;5(1):102794. Abstract Force generation is an essential property of skeletal muscle models in vitro. We describe a versatile 1-step procedure to direct undifferentiated human pluripotent stem cells (PSCs) into

Authors Lyra-Leite DM, Gutiérrez-Gutiérrez Ó, Wang M, Zhou Y, Cyganek L, Burridge PW Journal Star Protocols Citation STAR Protoc. 2022 Aug 18;3(3):101560. Abstract The methods for the culture and cardiomyocyte differentiation of human embryonic stem cells, and later human induced pluripotent stem cells (hiPSC), have moved from a complex and

Authors Sakib MS, Sokpor G, Nguyen HP, Fischer A, Tuoc T Journal Star Protocols Citation STAR Protoc. 2021 Feb 2;2(1):100318. Abstract Cell sorting can be used to purify cell populations for cell type-specific molecular probing. Fluorescence-activated cell sorting (FACS) coupled with high-throughput sequencing affords molecular signature identification for specific cell

Authors Tiburcy M, Meyer T, Liaw NY, Zimmermann WH Journal STAR protocols Citation STAR Protoc. 2020 Jun 3;1(1):100032. Abstract This protocol describes a robust method for the generation of engineered human myocardium (EHM) from pluripotent stem cells (PSCs) in a multi-well plate under defined, serum-free conditions. By parallel culture of