Nature Protocols

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Graphene- and metal-induced energy transfer for single-molecule imaging and live-cell nanoscopy with (sub)-nanometer axial resolution

Authors Ghosh A, Chizhik AI, Karedla N, Enderlein J Journal Nature Protocols Citation Nat Protoc (2021). Abstract Super-resolution fluorescence imaging that surpasses the classical optical resolution limit is widely utilized for resolving the spatial organization of biological structures at molecular length scales. In one example, single-molecule localization microscopy, the lateral
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Doubling the resolution of a confocal spinning-disk microscope using image scanning microscopy

Authors Qin S, Isbaner S, Gregor I, Enderlein J Journal Nature Protocols Citation Nat Protoc. 2020 Nov 27. Abstract Fluorescence microscopy has become an indispensable tool for cell biology. Recently, super-resolution methods have been developed to overcome the diffraction limit of light and have shown living cells in unprecedented detail.
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A mass spectrometry workflow for measuring protein turnover rates in vivo

Authors Alevra M, Mandad S, Ischebeck T, Urlaub H, Rizzoli SO, Fornasiero EF Journal Nature Protocols Citation Nat Protoc. 2019 Dec;14(12):3333-3365. Abstract Proteins are continually produced and degraded, to avoid the accumulation of old or damaged molecules and to maintain the efficiency of physiological processes. Despite its importance, protein turnover
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