Journal of Visualized Experiments

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20.08.2021

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications

Authors Santos GL, Meyer T, Tiburcy M, DeGrave A, Zimmermann WH, Lutz S Journal Journal of Visualized Experiments Citation J Vis Exp. 2021 Aug 20;(174). Abstract Fibroblasts are phenotypically highly dynamic cells, which quickly transdifferentiate into myofibroblasts in response to biochemical and biomechanical stimuli. The current understanding of fibrotic processes,
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22.12.2020

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Authors Seibertz F, Reynolds M, Voigt N Journal Journal of Visualized Experiments Citation J Vis Exp. 2020 Dec 22;(166). Abstract Conventional intracellular microelectrode techniques to quantify cardiomyocyte electrophysiology are extremely complex, labor intensive, and typically carried out in low throughput. Rapid and ongoing expansion of induced pluripotent stem cell (iPSC)
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03.11.2020

Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current

Authors Tomsits P, Schüttler D, Kääb S, Clauss S, Voigt N Journal Journal of Visualized Experiments Citation J Vis Exp. 2020 Nov 3;(165). Abstract Mouse models play a crucial role in arrhythmia research and allow studying key mechanisms of arrhythmogenesis including altered ion channel function and calcium handling. For this
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